Dezdelo prodaja 

disinf-want
 
 
Disinfection affairs № 3 / 2021


CONTENTS

DISINFECTION

pp. 5-15

DOI: 10.35411/2076-457X-2021-3-5-15

Evaluation of the effectiveness of preventive disinfection measures based on a comprehensive analysis of the microbiome of service dogs‘ places of detention

I.O. Krylova1, O.A. Chetina2, R.R. Zhdanov1

1Perm Military Institute of the National Guard of the Russian Federation: 1 Gremyachiy Loп str., Perm, 614112, Russian Federation;
2Perm State National Research University: 15 Bukireva, Perm, 614990, Russian Federation.

The article presents the results of a qualitative and quantitative analysis of the microbiome of places where dogs of service breeds are kept, obtained under the conditions of rotation of medicines for an active substance. In the period from 2017 to 2021, dynamic observations were made to assess the effectiveness of preventive disinfection measures carried out in the cynological town. The object of the study was the micro-and mycoflora of more than 20 dog enclosures in two versions: before disinfection and after mechanical treatment and fine-drip irrigation with preparations recommended for disinfection of veterinary surveillance facilities. Using differential diagnostic nutrient media, representatives of different groups of microorganisms were isolated from the surfaces of inventory, poilushki, the floor and walls of the walking enclosures. A qualitative analysis of biodiversity revealed that microbial isolates are conditionally pathogenic microorganisms and can be potentially dangerous infectious agents. The results showed that disinfection measures were not sufficiently effective against the selected groups of microorganisms. Using elective nutrient media, cultures of yeast-like and mold-like micromycetes were isolated from the surfaces of walking enclosures. The analysis of the data showed that both decompositions, claimed to be effective against mold micromycetes, do not withstand the contaminating load. The method of small-drop irrigation used for processing the walking of the kennels of the cynological town turned out to be low-effective in relation to the isolated microbial and fungal isolates. Within the framework of compliance with the sanitary and hygienic regime, an algorithm for organizing preventive disinfection measures in the cynological unit has been developed, based on the well-coordinated interaction of support services: medical, logistics, sanitary and veterinary. The research conducted jointly with representatives of the veterinary and sanitary service, with cadets of the cynological faculty, is of great practical importance for the formation of the professional competence of future officers – specialists-cynologists, heads of cynological units. The developed recommendations make it possible to adjust the algorithm of sanitary and hygienic measures in the cynological town and are of great importance in solving health-saving issues and maintaining the number of dogs of service breeds.
Keywords: preventive disinfection; sanitary-indicative microorganisms; micromycetes; health care of dogs; cynologists.

Authors
Krylova Irina Olegovna – PhD in biology, professor of the Department of Biology, Faculty of Cynological Perm Military Institute of the National Guard, s Forces of the Russian Federation; Tel.: (902) 800-97-78, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID: 0000-0002-7731-5225
Chetina Oksana Aleksandrovna – PhD in biology, associate Professor of the Department of Plant Physiology and Soil Ecology of the Faculty of Biology of the FSBEI VO PGNIU. ORCID ID: 0000-0002-6546-9753
Zhdanov Ruslan Radikovich – PhD in biology, head of the veterinary and sanitary service Department of the Perm Military Institute of the National Guard, s Forces of the Russian Federation. ORCID ID: 0000-0002-5551-2754

 

pp. 16-28

DOI: 10.35411/2076-457X-2021-3-16-28

Possible mechanisms of the destruction of spores of the vaccine strain Bacillus anthracis STI-1 under the influence of alcohol sols of metal nanoparticles

G.A. Frolov1, N.V. Zavyalova2, I.A. Lundovskikh3, M.R. Shabalina3, I.P. Pogorelsky4, K.I. Gurin5, A.V. Mironin5

1Federal State Autonomous Educational Institution of Higher Education "National Research Technological University: Moscow Institute of Steel and Alloys": 4 Leninsky Ave., Moscow, 119991, Russian Federation;
2Federal State Budgetary Institution "27 Scientific Center" of the Ministry of Defense of the Russian Federation: 13 Brigadirsky per., Moscow, 105005, Russian Federation;
3FSBEI HE "Vyatka State University": 36 Moskovskaya str., Kirov, 610000, Russian Federation;
4ANO "Scientific Society "Microbiota": Loza, 9 D, Sergiev Posad district, Moscow region, 141300, Russian Federation;
5Branch of the Federal State Budgetary Institution "48 Central Research Institute" of the Ministry of Defense of the Russian Federation: 121 Oktyabrsky Ave, Kirov, 610017, Russian Federation.

The results are presented of a comparative study of the sporicidal activity of alcohol sols of nanoparticles of titanium, copper, zinc and tantalum metals in the course of their interaction with a rehydrated lyophilized culture of spores of the vaccine strain Bacillus anthracis STI-1 with the loss of the ability of spores to germinate in nutrient media under optimal conditions using bacteriology and electronic microscopy. The most pronounced sporicidal effect is observed when titanium alcohol sol is exposed to spores. At the same time, spores of B. anthracis STI-1 in titanium alcohol sol are affected by ethyl alcohol, which causes dehydration and denaturation of the protein, the antiseptic cetylpyridinium chloride as a surfactant, which is also a strong electrophilic agent that destroys the exosporium and spore shells, and titanium nanoparticles, which, due to the high surface energy when exposed to spores, cause their pronounced massive adhesion. In addition, titanium nanoparticles, the zeta (ζ -) value of the potential of which is -44.5 mV, cause a stable energy state of the dispersed system of alcohol sol, which acts as a biocatalyst for intracellular enzymes, causing the hydrolysis of polymer structures of the spore shells and cortex, as well as destroyed nucleoid – an area of spores containing structured genetic material. Spores that do not have a nucleoid in electron microscopy acquire the form of "shadows". As a result of the action of titanium alcohol sol, the activation of germination and the formation of the so-called "metabolic" spores, from which the vegetative cells of B. anthracis STI-1 should have been formed, are prevented. The reason for the high sporicidal efficiency of the alcohol sol of titanium nanoparticles is associated both with the structure and properties and with the technology of synthesis of titanium nanoparticles in the low-temperature plasma zone with the formation, due to the excess of the surfactant cetylpyridinium chloride, of a two-layer shell around the metal nanoparticles, which leads to their "conservation" until the moment of contact with spores and a sharp slowdown in their oxidation in the aquatic environment. The destruction of the formed two-layer shell of metal nanoparticles begins with contact with spores with the simultaneous onset of the formation of titanium ions from the phase of nanoparticles, which actively destroy chemically the shells of the spores.
Keywords: microorganisms, Bacillus anthracis STI-1, spores, metal nanoparticles, zeta potential, disinfectant compositions, sporicidal activity.

Authors
Frolov Georgiy Aleksandrovich – Candidate of Chemistry Sciences, Associate Professor of Physical Chemistry of Moscow institute of steel and alloys; Tel.: (495) 955-00-03, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID: 0000–0003–1460–6030
Zavyalova Natalya Vasilevna – Doctor of Biological Sciences, Professor, Chief Researcher of 27 Scientific Center" of the Ministry of Defense of the Russian Federation
Lundovskikh Irina Aleksandrovna – Candidate of Chemistry Sciences, Associate Professor of Department of Microbiology of Vyatka State University. ORCID ID: 0000–0003–4954–8870
Shabalina Marina Robertovna – Candidate of Technical Sciences, Associate Professor of Fundamental and Applied Mathematics, Institute of Mathematics and Information Systems of Vyatka State University. ORCID ID: 0000–0001–5389–3306
Pogorelskiy Ivan Petrovich – Doctor of Medical Sciences, Professor, ANO "Scientific Society "Microbiota"
Gurin Konstantin Igorevich – Candidate of Medical Sciences, Chief of Research Center of 48 Central Scientific Research Institute
Mironin Aleksandr Viktorovich – Doctor of Medical Sciences, Professor, Leading Researcher of Research Center of 48 Central Scientific Research Institute

 

pp. 28-35

DOI: 10.35411/2076-457X-2021-3-28-35

Sensitivity of coronaviruses to disinfectants according to the results of foreign and domestic studies

L.G. Panteleeva

Scientific Research Disinfectology Institute of Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing: 18 Nauchny proezd, Moscow, 117246, Russian Federation.

More than 40 different species of the family Coronaviridae are known. The greatest epidemiological significance was acquired by SARS-CoV-2, the causative agent of a new coronavirus infection-COVID-19. It can persist on the surfaces of various objects from several hours to several days. It is sensitive to disinfectants from different chemical groups: chlorine-, oxygen-active, cationic surfactants, alcohols, aldehydes, and iodine-containing agents.
Keywords: coronaviruses, survival rate, disinfectants, virucidal activity, virus sensitivity.

Authors
Panteleeva Lyudmila Grigoryevna – Candidate of Medical Sciences, Сhief Researcher in Scientific Research Disinfectology Institute; Tel.: (495) 332-01-73, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript. ID: 0000-0002-9470-1750

 

pp. 36-39

DOI: 10.35411/2076-457X-2021-3-36-39

Safety assessment of residual amounts of disinfectants based on quaternary ammonium compounds on medical devices

A.L. Karaev, M.V. Bidevkina, M.V. Matrosenko

Scientific Research Disinfectology Institute of Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing: 18 Nauchny proezd, Moscow, 117246, Russian Federation.

Has been made an assessment of the toxicity of residual amounts of disinfectants after using them on products, made from various materials, with DS solutions based on QAC. The working solutions were used in concentrations from 0.3 to 7 % (by DV). The safety of the studied samples was assessed by the toxicity index "It" (in%) on an AT-05 Image Analyzer. Washing out of the residual amounts of DS in concentrations of 0.3–0.7 % (by DW) did not significantly affect the total time of disinfection treatment or pre-sterilization cleaning. At higher concentrations of solutions, there were differences in the time of washing test items and dishes for receiving safe amounts of DS. It is concluded that it is inexpedient to use solutions based on QAC in a concentration of more than 4.5 % (in terms of DV) for disinfection treatment and pre-sterilization cleaning of products made of natural and silicone rubber.
Keywords: disinfectants, quaternary ammonium compounds, product washing, AT-05 image analyzer, to­xicity index.

Authors
Karaev Andrey Lvovich – Candidate of Medical Sciences, Senior Researcher of Laboratory Concerning Toxicology in Scientific Research Disinfectology Institute; Tel.: (495) 332-01-56, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID: 0000-0001-8764-5159
Bidevkina Marina Vasilevna – Doctor of Medical Science, Leading Researcher – Head of Laboratory Concerning Toxicology in Scientific Research Disinfectology Institute. ORCID ID: 0000-0001-6433-899X
Matrosenko Margarita Vladimirovna – Junior Researcher of Laboratory Concerning Toxicology in Scientific Research Disinfectology Institute. ORCID ID: 0000-0003-0567-2247

 

pp. 40-48

DOI: 10.35411/2076-457X-2021-3-40-48

Evaluation of the effectiveness of chemical disinfection agents against the Epstein-Barr virus

T.V. Solomay1,2, E.I. Isaeva3, E.N. Vetrova3, A.I. Chernyshova3, T.A. Semenenko3,4, L.G. Panteleyeva5

1Interregional Department No. 1 of the FMBA of Russia: 6 1-y Pekhotniy pereulok, bld. 2, Moscow, 123182, Russian Federation;
2FGBNU "I.I. Mechnikov Scientific Research Institute of Vaccines and Serums" of the Ministry of Education and Science of the Russian Federation: 5а Maliy Kazenniy pereulok, Moscow, 105064, Russian Federation;
3FGBU "National Research Center of Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya" of the Ministry of Health of the Russian Federation: 18 Gamaleya str., Moscow, 123098, Russian Federation;
4I.M. Sechenov First Moscow State Medical University (Sechenov University): 8 Trubetskaya str., bld. 2, Moscow, 119991, Russian Federation;
5Scientific Research Disinfectology Institute of Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing: 18 Nauchny proezd, Moscow, 117246, Russian Federation.

The widespread spread of infection caused by the Epstein-Barr virus (EBV), the lack of registered vaccines, the possibility of implementing a transmission mechanism in medical organizations, determine disinfection as the main method of preventing this disease. The aim is to evaluate the effectiveness of chemical disinfection agents approved for use in the Russian Federation in relation to EBV-infected cell culture with quantitative DNA determination by polymerase chain reaction. Working solutions of complex disinfectants used for the treatment of medical devices were introduced into samples of EBV-infected B-cell culture in the concentration and exposure specified in the instructions (0.1 %; 60 min.). Then, the quantitative determination of the EBV DNA was performed. Chlorine-containing disinfectants, combined preparations based on aldehydes and guanidines per hour, as well as guanidines per hour and triamines, provided complete destruction of genetic material, while the combination of H with triamines led only to a decrease in the concentration of EBV DNA from 5.0 · 106 copies/ml to 2.4 · 106 copies/ml (by 2.1 times), which may not be enough to fully ensure biological safety when processing medical devices (sensors of ultrasound diagnostics devices, etc.), which are not subject to sterilization. The inability to determine the viability of EBV after treatment with disinfectants due to the initial presence of the virus in the B-cell culture makes the molecular biological method the only acceptable one at this stage and requires the search for new models – cell cultures intact to EBV or biological objects (laboratory animals).
Keywords: Epstein-Barr virus, disinfection, quaternary ammonium compounds, triamines, aldehydes, guanidines, active chlorine releasing agents.

Authors
Solomay Tatiana Valerievna – candidate of medical Sciences, Deputy head of the Interregional Department No. 1 of the FMBA of Russia; senior researcher, laboratory and epidemiological analysis and monitoring of infectious diseases, Federal state budgetary scientific institution "Scientific research Institute of vaccines and serums them. I.I. Mechnikov" of the Ministry of education of Russia; Tel.: (499) 190-39-90, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID: 0000-0002-7040-7653
Isayeva Elena Ivanovna – Candidate of Biological Sciences, Leading Researcher, Laboratory of Immunology, D.I. Ivanovsky Research Institute of Virology, Federal State Budgetary Institution "National Research Center of Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya". ORCID ID: 0000-0002-2523-0692
Vetrova Elizaveta Nikolaevna – Researcher of Laboratory of Immunology in D.I. Ivanovsky Research Institute of Virology of Federal State Budgetary Institution "National Research Center of Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya". ORCID ID: 0000-0003-1902-5278
Chernyshova Alyona Igorevna – Junior Researcher, Laboratory of Immunology, in D.I. Ivanovsky Research Institute of Virology of Federal State Budgetary Institution "National Research Center of Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya". ORCID ID: 0000-0003-1290-4042
Semenenko Tatyana Anatolievna – Doctor of Medical Sciences, Professor, Head of the Department of Epidemiology of FSBI "National Research Center of Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya"; Professor of the Department of Infectology and Virology of the IPO of the I.M. Sechenov First Moscow State Medical University. ORCID ID: 0000-0002-6686-9011
Panteleyeva Lyudmila Grigoryevna – Candidate of Medical Sciences, Associate Professor, Chief Researcher of Scientific Research Disinfectology Institute. ORCID ID: 0000-0002-9470-1750

 

DISINSECTION 

pp. 49-54

DOI: 10.35411/2076-457X-2021-3-49-54

Efficacy of chlorfenapyr- and hydramethylnon-based baits on laboratory colonies of the pharaoh ant

T.A. Davlianidze, O.Yu.Eremina, V.V. Olifer

Scientific Research Disinfectology Institute of Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing: 18 Nauchny proezd, Moscow, 117246, Russian Federation.

The effectiveness of insecticidal baits based on chlorfenapyr and hydramethylnone against colonies of the Pharaoh ant has been studied. Sugar baits containing 0.1–1.0 % chlorfenapyr are ineffective because after 4 weeks of the experiment, the viability of the colonies was not changed. Sugar baits based on 1 % hydramethylnon effectively destroyed workers, the effect on queens was weak, and depletion of the colony was achieved. Commercially produced baits effectively and completely destroy ant colonies both in the absence and in the presence of alternative food.
Keywords: Pharaoh ant, baits, chlorfenapyr, hydramethylnon.

Authors
Davlianidze Tatyana Alekseevna – Laboratory Research Assistant of Laboratory of Problems of Disinsection of Scientific Research Disinfectology Institute; Tel.: (495) 332-01-51, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID 0000-0002-2757-881X
Eremina Olga Yurevna – Doctor of Science in Biology; Leading Researcher of Laboratory of Problems of Disinsection of Scientific Research Disinfectology Institute. ORCID ID: 0000-0002-8268-2230
Olifer Veronika Valentinovna – Candidate of Science in Biology; Leading Researcher of Laboratory of Problems of Disinsection of Scientific Research Disinfectology Institute. ORCID ID:0000-0002-4736-7905

 

pp. 55-62

DOI: 10.35411/2076-457X-2021-3-55-62

Study of the efficiency of baits based on proinsecticides in relation to susceptible laboratory culture of pharaoh ants

T.A. Davlianidze, O.Yu. Eremina, V.V. Olifer

Scientific Research Disinfectology Institute of Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing: 18 Nauchny proezd, Moscow, 117246, Russian Federation.

The insecticidal properties of proinsecticides fipronil, indoxacarb, and methomyl were studied against the colonies of Pharaoh ant. It was found that sugar food baits based on fipronil or indoxacarb are effective against colonies of Pharaoh ant in the concentration ranges 0.001–0.1 % and 0.1–1.0 %, respectively. Baits based on 1 % methomyl are also highly effective. In the presence of alternative food, the mortality of workers and queens was reduced. The food attractiveness of ant baits should be increased by introducing honey, peanut butter, and other attractants.
Keywords: proinsecticides, fipronil, indoxacarb, methomyl, baits, Pharaoh ant.

Authors
Davlianidze Tatyana Alekseevna – Laboratory Research Assistant of Laboratory of Problems of Disinsection of Scientific Research Disinfectology Institute; Tel.: (495) 332-01-51, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID 0000-0002-2757-881X
Eremina Olga Yurevna – Doctor of Science in Biology; Leading Researcher of Laboratory of Problems of Disinsection of Scientific Research Disinfectology Institute. ORCID ID: 0000-0002-8268-2230
Olifer Veronika Valentinovna – Candidate of Science in Biology; Leading Researcher of Laboratory of Problems of Disinsection of Scientific Research Disinfectology Institute. ORCID ID:0000-0002-4736-7905

 

pp. 63-69

DOI: 10.35411/2076-457X-2021-3-63-68

Effect of insect growth regulators on two german cockroach strains and pharaoh ants

O.Yu. Eremina, V.V. Olifer

Scientific Research Disinfectology Institute of Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing:18 Nauchny proezd, Moscow, 117246, Russian Federation.

When eating poisoned baits based on 0.1–1.0 % pyriproxyfen, within 6 weeks killed up to 50 % of German cockroaches of the S-NIID strain, and 12 % of cockroaches of the OBN strain. The resulting oothecae turned out to be defective and the second generation of cockroaches did not hatch. Morphological changes consisted were in the deformation of the wings (rudimentary, curved, twisted) and in the inability of the larvae to transform into adults. In experiments with an alternative food, similar results were obtained, females formed more oothecae, but the emergence of new generation larvae was not recorded. The consumption of pyriproxyfen-based baits by Pharaoh ant led to the elimination of colonies. A significant decrease in brood number was recorded at 3–6 weeks, and complete death of brood was observed at 6–8 weeks in all treated colonies. The absence of brood can be explained by both mortality as a result of developmental disorders and the cessation of egg laying by queens.
Keywords: insect growth regulators, German cockroach, Pharaoh ant, pyriproxifen, hydroprene.

Authors
Eremina Olga Yurevna – Doctor of Science in Biology; Leading Researcher of Laboratory of Problems of Disinsection of Scientific Research Disinfectology Institute; Tel.: (495) 332-01-51, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID: 0000-0002-8268-2230
Olifer Veronika Valentinovna – Candidate of Science in Biology; Leading Researcher of Laboratory of Problems of Disinsection of Scientific Research Disinfectology Institute. ORCID ID:0000-0002-4736-7905
 

DISINFECTION BUSINESS MANAGEMENT

pp. 63-69

DOI: 10.35411/2076-457X-2021-3-69-73

Features of periodic accreditation of specialists in the specialties «Disinfection», «Parasitology» and other specialties in 2021

T.V. Gololobova1,2, T.I. Avdyukhina1, T.A. Semenova1, A.A. Androsov1, T.G. Suranova2

1Russian Medical Academy of Continuing Professional Education of Russian Ministry of Health: 2/1 building 1 Barrikadnaya str., Moscow, 125993, Russian Federation;
2Scientific Research Disinfectology Institute of Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing: 18 Nauchny proezd, Moscow, 117246, Russian Federation.

The article highlights in detail the features of the periodic accreditation of specialists in 2021, gives detailed recommendations to doctors on the formation of an educational trajectory and on the preparation of documents for accreditation. The authors present the rules of operation of the Federal Accreditation Center, listed regulatory legal documents.
Keywords: primary accreditation of specialists, professional development programs, portfolio, Department of Parasitology and Disinfection.

Authors
Gololobova Tatyana Viktorovna – Doctor of Medical Science, Acting Director of Scientific Research Disinfectology Institute, Department Chair of Parasitology and Disinfection of Russian Medical Academy of Continuing Professional Education; Tel.: (495) 332-01-01, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.
Avdyukhina Tatyana Ivanovna – Candidate of Medical Science, Professor of Parasitology and Disinfection of Russian Medical Academy of Continuing Professional Education
Semenova Tatyana Aleksandrovna – Candidate of Science in Biology, Associate Professor of Parasitology and Disinfection of Russian Medical Academy of Continuing Professional Education
Androsov Andrey Anatolevich – Candidate of Medical Science, Associate Professor of Parasitology and Disinfection of Russian Medical Academy of Continuing Professional Education
Suranova Tatyana Grigorevna – Candidate of Medical Science, Chief Researcher of Scientific Research Disinfectology Institute

Life of National Organization of Disinfectionists (NOD)

p. 72

Congratulation new members of NOD

 

OBITUARY

p. 73

Rumyantseva L.N. (1949–2021)

 

INFORMATION

p. 74

Subscription to journal "Disinfection Affairs"