DISINFECTION
pp. 5-23
DOI: 10.35411/2076-457X-2023-1-5-23
Experimental evaluation of Yersinia pseudotuberculosis bacteria sensitivity to antimicrobial compositions based on copper nanoparticles in in vitro and in vivo experiments
G.A. Frolov1, A.A. Abdukadirov ugli1, I.A. Lundovskikh2, A.Yu. Mironina2, K.I. Gurin3, I.P. Pogorelsky3, A.V. Mironin3, K.E. Gavrilov3
1National University of Science and Technology MISIS: 4 Leninsky Ave., Moscow, 119991, Russian Federation;
2Vyatka State University: 36 Moskovskaya St., Kirov, 610000, Russian Federation;
3Branch of the Federal State Budgetary Establishment 48 Central Research Institute of the Ministry of Defense of the Russian Federation: 121 Oktyabrsky Ave, Kirov, 610017, Russian Federation.
The paper presents the results of the antibacterial efficacy of disinfecting compositions based on copper nanoparticles and its oxides in antiseptic solutions of cetylpyridinium chloride and benzalkonium chloride against the causative agent of pseudotuberculosis Y. pseudotuberculosis of in vitro and in vivo experiments. Using the bacteriological method and the results of electron microscopy, high bactericidal activity of disinfecting compositions has been proven. Anti-yersiniosis activity and safety of both compositions were revealed in experimental pseudotuberculosis of conventional white mice. Simultaneous oral administration of the compositions under study to experimental animals limits the initiation and development of the infectious process and prevents the death of laboratory animals. Possible mechanisms of enhancing the biocidal activity of the tested disinfecting compositions are considered.
Keywords: pseudotuberculosis, microorganisms, biocidal activity, antiseptic, nanoparticles, zeta potential.
Authors
Frolov Georgiy Aleksandrovich – Candidate of Chemical Sciences, Associate Professor of Physical Chemistry Department, National University of Science and Technology MISIS. ORCID ID: 0000-0003-1460-6030
Abdukadirov ugli Abdulla Abumalik – Postgraduate Student of Physical Chemistry Department, National University of Science and Technology MISIS
Lundovskikh Irina Aleksandrovna – Candidate of Chemical Sciences, Associate Professor of Microbiology Department, Vyatka State University; Еel.: (8332) 74-24-40, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript. (the corresponding author). ORCID ID: 0000-0003-4954-8870
Mironina Anna Yuryevna – Candidate of Philological Sciences, Associate Professor of Linguistics and Translation Department, Vyatka State University. ORCID ID: 0000-0003-0448-2812
Gurin Konstantin Igorevich – Candidate of Medical Sciences, Deputy Director of Research Center, 48 Central Research Institute of the Ministry of Defense of the Russian Federation
Pogorelsky Ivan Petrovich – Doctor of Medical Sciences, Professor, Leading Researcher, 48 Central Research Institute of the Ministry of Defense of the Russian Federation. ORCID ID: 0000-0001-6293-7366
Mironin Aleksandr Victorovich – Doctor of Medical Sciences, Leading Researcher, 48 Central Research Institute of the Ministry of Defense of the Russian Federation. ORCID ID: 0000-0002-2434-2385
Gavrilov Konstantin Evgenyevich – Candidate of Biological Sciences, Researcher, 48 Central Research Institute of the Ministry of Defense of the Russian Federation
pp. 23-30
DOI: 10.35411/2076-457X-2023-1-23-30
Safety of using disinfectants based on sodium hypochlorite in practice
G.P. Pankratova, M.V. Bidevkina, Z.К. Shaikhutdinova
Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F.F. Erisman: 2 Semashko st., Mytishchi, Moscow region, 141014, Russian Federation.
The parameters of toxicometry of disinfectants based on sodium hypochlorite obtained by chlorination of an aqueous solution of caustic soda (31 agents) and by electrochemical method – 10 anoliths were established. Based on the determination of the chlorine content in the air, recommendations are given for the safe use of these disinfectants: for surface treatment by wiping and disinfection of air and surfaces by aerosol spraying using a special device – an aerosol generator that provides dispersion of aerosol particles of 5–50 microns. A working solution of sodium hypochlorite with an active chlorine content of 0.015 % is recommended for use by wiping in the presence of people.
Keywords: disinfectants, sodium hypochlorite, anolytes, toxicity, irritant effect, inhalation hazard, aerosol, laboratory animals.
Authors
Galina P. Pankratova – PhD in Medicine, Leading Researcher the Department of Toxicology Disinfectology institute of the FSUE "FNTSG named after F.F. Erisman" of Rospotrebnadzor; e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript., Tel.: (916) 705-18-98. ORCID 0000-0003-2471-7906
Marina V. Bidevkina – Doctor of in Medicine, Head of the Department of Toxicology Disinfectology institute of the FSUE "FNTSG named after F.F. Erisman" of Rospotrebnadzor. ORCID 0000-0001-6433-899X
Zukhra К. Shaikhutdinova – Junior researcher of the Department of Toxicology of the Institute of Disinfection of the FSUE "FNTSG named after F.F. Erisman" of Rospotrebnadzor. ORCID ID 0000-0002-6364-9603
pp. 30-40
DOI: 10.35411/2076-457X-2023-1-30-40
Direct and combined effects of ozone on biofilm Pseudomonas aeruginosa
A.A. Ushakova, I.F. Karimov, S.D. Borisov, A.S. Pankov, A.G. Korneev
Orenburg State Medical University (OrSMU):6 Sovetckaya str., Orenburg, 460000, Russian Federation.
In modern medicine, the issue of combating biofilms, including various surfaces in medical facilities, is acute. Ozonation is an environmentally friendly and effective technology in the fight against microbial pollution, including biofilms. The article presents studies of the disinfecting properties of ozone and its combination with various disinfectants on planktonic and adhered (in the biofilm form) Pseudomonas aeruginosa cells. As a result, it was found that ozone is highly effective against various forms of bacterial life, and also requires longer exposure in the presence of organic pollution. When combined with hydrogen peroxide, it shows the greatest bactericidal effect not only on the destruction of the biofilm, but also on the viability of the cells themselves.
Keywords: biofilm, ozone disinfection, destruction of biofilm.
Authors
Ushakova Anastasiya Alekseevna – Assistant of the Department of Epidemiology and Infectious Diseases of OrSMU; Tel.: (922) 559-55-69, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID: 0000-0002-9882-5726
Karimov Ilshat Fayzelgayanovich – Candidate of Biological Sciences, Biologist of the Microbiological Laboratory of the Research Center, Associate Professor of the Department of Microbiology, Virology and Immunology of OrSMU. ORCID ID: 0000-0001-6853-4242
Borisov Sergey Dilyusovich – Candidate of Medical Sciences, Head of the Microbiological Laboratory of the Research Center, Associate Professor of the Department of Microbiology, Virology and Immunology of OrSMU. ORCID ID: 0000-0002-4386-8745
Pankov Aleksandr Sergeevich – Associate Professor, Doctor of Medical Sciences, Head. Department of Epidemiology and Infectious Diseases of OrSMU. ORCID ID: 0000-0003-4994-6633
Korneev Aleksey Gennadevich – Candidate of Biological Sciences, Associate Professor of Epidemiology and Infectious Diseases of OrSMU. ORCID ID: 0000-0002-7574-0527
DISINSECTION
pp. 41-46
DOI: 10.35411/2076-457X-2023-1-41-46
Reversion of susceptibility to insecticides of male German cockroach of resistant strains during long-term cultivation under laboratory conditions
O.Yu. Eremina1, V.V. Olifer1, T.A. Davlianidze2
1Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F.F. Erisman: 2 Semashko st., Mytishchi, Moscow region, 141014, Russian Federation;
2FBUN "Research Institute of Systems Biology and Medicine" of Rospotrebnadzor: 18 Nauchny proezd, Moscow, 117246, Russian Federation.
Under laboratory conditions, resistance to insecticides from various chemical classes was assessed on several strains of the German cockroach Blattella germanica obtained from populations collected at sites in Moscow, Kaluga, Sverdlovsk and Moscow regions. It has been established that when applied topically, German cockroaches of all strains are highly resistant to cypermethrin (RR = 133–>4000×), fipronil (RR = 14–61×), chlorpyrifos (RR = 15–40×), weakly tolerant to thiamethoxam and clothianidin (RR = 2–5×). After breeding in an insectarium without insecticide press for several generations, the resistance of M1 strain to cypermethrin decreased by 1.2 times, in U1, M9 and OBN strains resistance remained at the same high level, in U8 strain decreased by 20 times; the resistance to fipronil in M1, U1 and OBN strains decreased by 1.4, 1.7 and 5.0 times, respectively. The level of susceptibility to chlorpyrifos decreased by 1.2–2.2 times, depending on the strain. Tolerance of cockroaches to thiamethoxam and clothianidin remained at the same level. The mechanisms of resistance of the German cockroach to insecticides are discussed.
Keywords: pyrethroids, neonicotinoids, organophosphorus compounds, phenylpyrazoles.
Authors
Eremina Olga Yurevna – Doctor of Science in Biology; Leading Researcher of the Division of disinsection of the Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F.F. Erisman; Tel.: (495) 332-01-51, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID: 000-0002-8268-2230
Olifer Veronika Valentinovna – Candidate of Science in Biology; Leading Researcher of the Division of disinsection of the Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F.F. Erisman. ORCID ID: 0000-0002-4736-7905
Davlianidze Tatyana Alekseevna – Laboratory Research Assistant of the Research Institute of Systems Biology and Medicine. ORCID ID 0000-0002-2757-881X
DISINFECTION PRACTICES
pp. 46-52
DOI: 10.35411/2076-457X-2023-1-46-52
Management of changes in the disinfection services industry
P.Yu. Bychkov1, E.O. Belov2
1State unitary enterprise of the city of Moscow "Moscow City Disinfection Center": 129337, Moscow, Yaroslavskoye Shosse, 9;
2LLC "PestControlGroup": 2 Nagornoye Shosse, bldg. 9A, Khimki, Moscow Region, 141407, Russian Federation.
On the example of the Moscow City Disinfection Center, issues of improving the quality of work and the effectiveness of the organization providing disinfection services at the current stage, professional training of specialists, finding new clients and retaining contractual relations are considered.
Keywords: disinfection, advanced training, marketing, quality of services.
Authors
Bychkov Pavel Yuryevich – Deputy Head for Medical Activities of the "Moscow City Disinfection Center"; Tel. (915) 072-71-41, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript..
Belov Evgeny Olegovich – General Director of PestControlGroup LLC
ORGANIZATION OF DISINFECTION AFFAIRS
pp. 52-55
DOI: 10.35411/2076-457X-2023-1-52-55
Experience in the practical use of a multifunctional disinfection station for the purpose of additional measures during fighter disinsection measures on the example of bed bugs
N.A. Vatolina1, A.M. Datskevich2, L.I. Kabay2, L.V. Lyalina1, T.V. Osmirko3
1FBUN "St. Petersburg Research Institute of Epidemiology and Microbiology named after Pasteur" of Rospotrebnadzor: 14 Mira st., St. Petersburg, 197101. Russian Federation;
2"St. Petersburg City Disinfection Station": 4b Finskiy per., St. Petersburg, 195009. Russian Federation;
3FGBOU VO "North-Western State Medical University named after I.I. Mechnikov": 41 Kirochnaya st., St. Petersburg, 191015, Russian Federation.
Today, disinfection institutions, having a small selection of insecticide active ingredients and a huge selection of mix insecticide preparations to combat bed bugs, face the problem of ensuring effective processing of objects of different categories.
Keywords: preventive disinfection, fighter disinsection measures, disinsection, chamber disinfection (disinsection), bed bugs, tropical bed bugs, disinsection equipment.
Authors
Vatolina Natalya Anatolevna – Expert in the Field of Certification "Organization and Conduct of Disinfectological Examinations, Assessments of Research Results, Measurements," Degree Applicant of St. Petersburg Research Institute of Epidemiology and Microbiology named after Pasteur; Tel: (981) 944-61-33 , e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.
Datskevich Aleksey Mikhaylovich – Head of the Preventive Ddisinfection Department, Disinfectologist of the Highest Qualification Ccategory of St. Petersburg City Disinfection Station
Kabay Lyubov Ivanovna – Zoologist (Entomologist) of the Preventive Disinfection Department of St. Petersburg City Disinfection Station
Lyalina Lyudmila Vladimirovna – Doctor of Medical Sciences, Professor, Head of the Laboratory of Epidemiology of Infectious and Non-Communicable Diseases of St. Petersburg Research Institute of Epidemiology and Microbiology named after Pasteur
Osmirko Tatyana Vladimirovna – Associate Professor, Candidate of Medical Sciences, Department of Epidemiology, Parasitology and Disinfectology of North-Western State Medical University named after I.I. Mechnikov
FOREIGN EXPERIENCE
pp. 56-62
DOI: 10.35411/2076-457X-2023-1-56-62
Synthesis and study of a disinfectant based on diethanolamine
V.M. Farzaliev, M.T. Abbasova, N.A. Alieva, L.R. Safarova, G.M. Kulieva
Institute of Chemistry of Additives, named by academician A.M. Guliev of the Ministry of Science and Education of the Republic of Azerbaijan: 2062 Beyukshor av., Baku, Az-1029, Azerbaijan.
The interaction of diethanolamine with formaldehyde at a ratio of initial reagents of 2 : 3, respectively, obtained a preparation that is an equimolar mixture of 3-(β-hydroxyethyl)-1,3-oxazolidine and its methylol derivative – 3-(β-hydroxyoxymethyl-ethyl)-1,3-oxazolidine. The paper presents the results of a study of the antimicrobial activity of the preparation against microorganisms – S. aureus, E. coli, as the most resistant species of bacteria that cause nosocomial infection. The tests were carried out by three methods: the test object method, as well as suspension and adsorption methods. The minimum effective concentration and exposure time of the preparation, which ensure the complete destruction of these microorganisms, have been established. The effect of organic substances and neutralizers on the antimicrobial activity of the preparation was studied. "Sulfonol" (a mixture of stereoisomers of the sodium salt of alkylbenzenesulfonic acid) at a concentration of 0,2 % and ten percent milk were used as neutralizers, and 10 % horse serum was used as protein. The concentration of the preparation and the exposure time required for the complete disinfection of dishes, glass and metlakh tiles infected with S. aureus and E. coli were determined. Disinfection of surfaces is achieved by treatment with a 3 % solution of the preparation during an exposure of 60 minutes, and dishes – with 3–5 % solutions for 45 minutes. It has been established by the thermoanalytical method of analysis that the preparation is stable at normal temperature and does not change its physicochemical properties during storage for 18 months or more. The preparation is recommended as a disinfectant for medical purposes.
Keywords: 1,3-oxazolidine derivatives, 3-(β-hydroxyethyl)-1,3-oxazolidine, 3-(β-hydroxyoxymethylethyl)-1,3-oxazolidine, antimicrobial activity, bactericidal activity, nosocomial infection, bacteria, S. aureus, E. coli, disinfection, disinfectant.
Authors
Farzaliev Vagif Majid – Dr. of Chem. Sci., Academician, General Director of the Institute of Chemistry of Additives, named by academician A.M. Kuliev of the Ministry of Science and Education of the Republic of Azerbaijan; services phone/fax: (99412) 514-96-12, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript., ORCID ID: 0000-0002-5089-233X
Abbasova Malahat Talat – Ph.D. Chem. Sciences, Associate Professor, Leading Researcher. ORCID ID: 0000-0002-0582-4653
Nabiev Oruc Garib – Ph.D. Chem. Sciences, Associate Professor, Head of Laboratory. ORCID ID: 0000-0002-4656-8991
Alieva Nabat Allahverdi – Engineer. ORCID ID: 0000-0003-0463-3270
Safarova Leyla Ramiz – Senior Researcher. ORCID ID: 0000-0002-4373-9628
Kulieva Garatel Maharram – Senior Researcher. ORCID ID: 0000-0002-9972-5719
OBITUARY
pp. 62
Makhanko E.V. (1934–2023)
INFORMATION
pp. 63
List of materials published in journal "Disinfection Affairs" in 2022
Life of National Organization of Disinfectionists (NOD)
pp. 64
Congratulation new members of NOD