Life of National Organization of Disinfectionists (NOD)
p. 5
Congratulation new members of NOD
DISINFECTION
pp. 5-18
DOI: 10.35411/2076-457X-2020-4-5-18
Morphofunctional changes in Bacillus cereus cells under the influence of nanoparticles of metals and metal oxides
G.A. Frolov1, I.A. Lundovskikh2, M.R. Shabalina2, M.B. Tarasov3, I.P. Pogorelsky4, K.I. Gurin5, A.V. Mironin5
1National University of Science and Technology "Moscow institute of steel and alloys": 4 Leninsky Ave., Moscow, 119991, Russian Federation;
2Vyatka State University: 36 Moskovskaya str., Kirov, 610000, Russian Federation;
3LLC NPF "NanoTechProm": Koroleva St., A2, of. 60, Belgorod, 308004, Russian Federation;
4ANO "Scientific society "Microbiota": Loza, 9 D, Sergiev Posad district, Moscow region, 141300, Russia;
5Branch of the Federal State Budgetary Establishment "48 Central Scientific Research Institute" of the Ministry of Defense of the Russian Federation: 121 Oktyabrsky Ave, Kirov, 610017, Russian Federation.
The results are presented of the comparative study of the presence of characteristic features of disorders of the microstructural organization of the test strain B. cereus ATCC 10702 cells by stable nanoparticles of silver, gold, platinum, zinc oxide and titanium dioxide with loss of cell viability during their interaction with nanoparticles in an aqueous medium using methods of bacteriology and electron microscopy. General size-dependent features of the effect of nanoparticles on B. cereus ATCC 10702 cells and differences associated with both the adsorption of metal oxide and noble metal nanoparticles on the surface of bacteria and the loss of viability of bacterial cells with different levels of their destruction, reaching 67-70 % in the case of nanoparticles noble metals, were found. The chemistry of the effects of silver, gold, platinum and zinc and titanium oxides nanoparticles on the test microbe B. cereus ATCC 10702 is considered. The assumption is made that the main feature of the interaction of nanodispersed structures based on noble metals and metal oxides is not associated with an increase in the specific contact surface of the dispersed phase, but with a significant increase in the specific surface energy, which leads to the modification of the multilayer hydrated water shells surrounding nanoparticles. The measured zeta (ζ-)-potential of metal and metal oxides nanoparticles characterizes the total effect formed on the diffusion-mobile boundary of the nanoparticle; its value is a quantitative characteristic of the equilibrium energy state of dispersed systems acting as biocatalysts of intracellular enzymes and causing hydrolysis of the polymer structures of cytoplasmic membranes and cell membranes and ultimately the death of microbial cells. The obtained experimental data can be used in creation effective antimicrobial compositions.
Keywords: microorganisms, Bacillus cereus АТСС 10702, biocidal activity, nanoparticles, zeta potential, disinfectant compositions.
AUTHORS
Frolov Georgiy Aleksandrovich – Candidate of Chemistry Sciences, Associate Professor of Physical Chemistry of Moscow institute of steel and alloys; Tel.: (495) 955-00-03, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID: 0000-0003-1460-6030
Lundovskikh Irina Aleksandrovna – Candidate of Chemistry Sciences, Associate Professor of Department of Microbiology of Vyatka State University. ORCID ID: 0000-0003-4954-8870
Tarasov Mikhail Borisovich – General Director of LLC " NPF NanTekhProm". ORCID ID: 0000-0003-0980-5908
Shabalina Marina Robertovna – Candidate of Technical Sciences, Associate Professor of Fundamental and Applied Mathematics, Institute of Mathematics and Information Systems of Vyatka State University. ORCID ID: 0000-0001-5389-3306
Pogorelskiy Ivan Petrovich – Doctor of Medical Sciences, Professor, ANO "Scientific Society "Microbiota"
Gurin Konstantin Igorevich – Candidate of Medical Sciences, Chief of Research Center of 48 Central Scientific Research Institute
Mironin Aleksandr Viktorovich – Doctor of Medical Sciences, Professor, Leading Researcher of Research Center of 48 Central Scientific Research Institute
pp. 18-27
DOI: 10.35411/2076-457X-2020-4-18-27
Application of high performance liquid chromatography with a charged aerosol detector for the determination of quaternary ammonium compounds in disinfectants
D.A. Merkulova1,2, A.S. Ivanova1, Yu.A. Efimova2, A.D. Salimova1, S.V. Andreev1
1Scientific Research Disinfectology Institute of Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing: 18 Nauchny proezd, Moscow, 117246, Russian Federation;
2Institute of Fine Chemical Technologies of MIREA – Russian Technological University: 86 Vernadsky Avenue, Moscow, 119571, Russian Federation.
Cationic surfactants based disinfectants have been widely used in practice for over 20 years. Despite the significant advances in analytical chemistry during this period, the determination of mixtures of several quaternary ammonium compounds in one disinfectant still causes certain difficulties. This work describes the separation in the combined presence of alkyldimethylbenzylammonium chloride, alkyldimethyl (ethylbenzyl) ammonium chloride, and dimethyldioctylammonium chloride in disinfectants using high performance liquid chromatography with a charged aerosol detector. The method was tested on model solutions and real disinfectants. The linearity range for alkyldimethylbenzylammonium chloride was from 0.03 to 0.17 %, for alkyldimethyl (ethylbenzyl) ammonium chloride – from 0.03 to 0.15 %, and from 0.01 to 0.11 % for dimethyldioctylammonium chloride. The calculated relative error of the method did not exceed 9 %. Thus, the proposed method can be used to analyze preparative forms of disinfectants containing these and other cationic surfactants.
Keywords: alkyldimethylbenzylammonium chloride, alkyldimethyl (ethylbenzyl) ammonium chloride, dimethyldioctylammonium chloride, disinfectants, HPLC, charged aerosol detector.
AUTHORS
Daria A. Merkulova – Assistant Researcher of Chemical Department of Scientific Research Disinfectology Institute, student of Institute of Fine Chemical Technologies of MIREA, Tel.: (495) 332-01-07, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID: 0000-0002-5021-2349;
Anastasia S. Ivanova – Junior Researcher of Chemical Department of Scientific Research Disinfectology Institute. ORCID ID: 0000-0002-1919-2810
Yulia A. Efimova – PhD in Chemistry, Docent of Institute of Fine Chemical Technologies of MIREA. ORCID ID: 0000-0001-7795-7490
Anna D. Salimova – Junior Researcher, Chemical Department of Scientific Research Disinfectology Institute. ORCID ID: 0000-0002-4634-6977
Sergey V. Andreev – PhD in Chemistry, Leading Researcher, Head of Chemical Department of Scientific Research Disinfectology Institute. ORCID ID: 0000-0003-2405-9931
pp. 27-40
DOI: 10.35411/2076-457X-2020-4-27-40
Retrospective analysis of developments on the use of sterilization packaging materials for medical devices (literature review)
G.N. Melnikova
Scientific Research Disinfectology Institute of Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing: 18 Nauchny proezd, Moscow, 117246, Russian Federation.
The article discusses issues related to the creation and use of packaging materials for different types of products, including those specially designed for placing medical devices in them, before sterilization. The changes taking place related to the improvement of the barrier properties of packages to extend the preservation period of sterility of medical devices (both new types and complex in design) sterilized by different methods are noted. It is shown how over the course of several years new materials have been developed, technologies/methods of processing and reprocessing of raw materials and materials have changed, which make it possible to obtain packaging materials of different chemical nature and properties. Changes in existing packages from traditional materials (metal) to of single-use sterilization packages of a new generation with improved manufacturing technologies that provide mechanical, barrier and antimicrobial properties of packages differing in types, sizes, material properties are noted. The priority in the use of packaging materials for medical devices remains for materials that meet the requirements of GOST ISO 11607 "Packaging for medical devices subject to terminal sterilization", since the standard imposes more stringent requirements for the execution of packaging materials.
Keywords: sterilization packaging materials, sterilization boxes, medical devices, sterilization, sterilization methods, preservation of sterility.
AUTHORS
Melnikova Galina Nikolaevna – Candidate of Medical Sciences, Leading Researcher of Department of Organization and Support of Scientific and Educational Activities of Scientific Research Disinfectology Institute; Tel.: (499) 147-46-85, email: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID: 0000-0002-4471-7420
pp. 41-50
DOI: 10.35411/2076-457X-2020-4-41-50
Modern devices for treatment of the surgeon’s hands and the surgical field (literature review)
A.M. Morozov, A.N. Sergeev, G.A. Dubatolov, N.A. Sergeev, S.V. Zhukov, T.S. Ryzhova, K.I. Khorak
Federal State Budgetary Educational Institution of Higher Education "Tver State Medical University" of Ministry of Healthcare of the Russian Federation: 4 Sovetskaya str., Tver, 170000, Russian Federation.
The aim – analyze modern Russian and foreign literary sources in order to determine modern means for treating the hands of the surgeon and the operating field. Results. One of the key points in the prevention of surgical infection is the treatment of the surgeon’s hands and the operating field with effective skin antiseptics in order to destroy pathogenic and opportunistic microorganisms that colonize intact skin. In modern practice, skin antiseptics are predominantly used containing alcohols as active substances, in particular ethyl, propyl and isopropyl, halogenated substances such as iodine and iodophores, guanidines, which include chlorhexidine digluconate, as well as quaternary ammonium compounds. Moreover, the most widespread are combined preparations containing several active substances and functional additives, which makes it possible to neutralize the negative properties of various active substances. Also, an interesting and promising direction is the use of polymer operating films or film-forming antiseptics. Currently, research is being actively carried out aimed at finding and developing modern highly effective antiseptic agents and their rational combinations that meet the necessary requirements, are optimal in their properties, cost-effective and comfortable to use.
Keywords: skin antiseptics, asepsis and antiseptics, surgical treatment of hands, treatment of the operating field.
AUTHORS
Morozov Artem Mikhailovich – Candidate of Medical Sciences, Associate Professor at the Department of General Surgery of FSBEI HE Tver SMU MOH Russia; Tel.: (4822) 55-45-55, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID: 0000-0003-4213-5379
Sergeev Alexey Nikolaevich – Doctor of Medical Sciences, Associate Professor, Head of the Department of General Surgery of FSBEI HE Tver SMU MOH Russia. ORCID ID: 0000-0002-9657-8063
Dubatolov Gennady Alexandrovich – Candidate of Medical Sciences, Associate Professor at the Department of General Surgery of FSBEI HE Tver SMU MOH Russia
Sergeev Nikolay Alexandrovich – Doctor of Medical Sciences, Professor at the Department of General Surgery of FSBEI HE Tver SMU MOH Russia ORCID ID: 0000-0002-3459-2863.
Zhukov Sergey Vladimirovich – Doctor of Medical Sciences, Professor of the Department of Public Health and Health Care of FSBEI HE Tver SMU MOH Russia. ORCID ID: 0000-0002-3145-9776
Ryzhova Taisia Stanislavovna – student of FSBEI HE Tver SMU MOH Russia. ORCID ID: 0000-0002-8572-4862
Khorak Konstantin Iosifovich – student of FSBEI HE Tver SMU MOH Russia. ORCID ID: 0000-0003-4043-4164
DISINSECTION
pp. 51-59
DOI: 10.35411/2076-457X-2020-4-51-59
New insecticide in medical disinsection – indoxacarb (oxadiazins)
O.Yu. Eremina, T.A. Davlianidze
Scientific Research Disinfectology Institute of Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing: 18 Nauchny proezd, Moscow, 117246, Russian Federation.
A review of foreign literature is devoted to the insecticide indoxacarb from the group of oxadiazines. Information on the preparations used and the concentrations of indoxacarb in relation to synanthropic insects is given. The mechanism of action, biotransformation, insect resistance to indoxacarb are considered.
Keywords: indoxacarb, oxadiazines, cockroaches, houseflies, mosquitoes, resistance.
AUTHORS
Eremina Olga Yurevna – Doctor of Science in Biology; Leading Researcher of Laboratory of Problems of Disinsection of Scientific Research Disinfectology Institute; Tel.: (495) 332-01-51, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. Orcid ID 0000-0002-8268-2230
Davlianidze Tatyana Alekseevna – Laboratory Research Assistant of Laboratory of Problems of Disinsection of Scientific Research Disinfectology Institute. Orcid ID 0000-0002-2757-881X
DERATIZATION
pp. 59-70
DOI: 10.35411/2076-457X-2020-4-59-70
Prevention of human infections with hemorrhagic fever with renal syndrome in the Saratov region
A.N. Matrosov1, A.A. Kuznetsov1, A.V. Ivanova1, N.M. Ermakov1, U.A. Chumachkova1, S.I. Tolokonnikova1, U.A. Kresova2, M.A. Tarasov2, R.A. Romanov2, V.K. Sonin2, A.N. Dzyabko2, N.I. Matveeva3, G.N. Arkhipova3, I.N. Vyatkin3, N.V. Popov1, O.I. Kozhanova3
1Russian Research Anti-Plague Institute "Microbe" of the Rospotrebnadzor: 46 Universitetskaya str., Saratov, 410005, Russian Federation;
2FBHI "Center of Hygiene and Epidemiology in the Saratov Region": 69 Bolshaya Gornaya str., Saratov, 410031, Russian Federation;
3Rospotrebnadzor Department in the Saratov region: 7 Volskaya str., Saratov, 410028, Russian Federation.
The territory of the Saratov Region is situated predominantly in steppe area and is not among the constituent entities of the Russian Federation characterized by consistently high human infections with hemorrhagic fever (HFRS) incidence rates among the population. In certain years, extreme weather events in combination with good feeding and protective conditions result in hard to forecast outbreaks of the red vole numbers – main carrier of the hantavirus Puumala. Risk territories for intensive and extensive epizooty development are intra-zonal and azonal forest biotopes, to which recreation, residential areas and dacha settlements adjust. Epidemic complications on HFRS have been registered since 1960s. The outbreaks of the disease occurred in 1986, 1992, 1999, and 2014. Record-breaking as regards the number of cases outbreak was reported in 2019, associated with intensive sub-snow reproduction of the red vole during winter season. Intensive morbidity rate was 109.7 per 100 thousand of the population, which is tens of times higher than the long term average annual values (7.2). Under the given circumstances, a broad range of preventive measures was implemented, including additional monitoring investigations, restrictive, and sanitary-technical activities, outreach campaign, disinfection and deratization. As a result of the measures performed, incidence rates in the first half of 2020 was reduced to 5.7 per 100 thousand of the population. The lessons learned can help to avoid similar events in the region, other constituent entities that are enzootic in terms of this zoonosis. The results obtained should also be taken into account when monitoring other zoonoses relevant to the region, as well as on the territories of other subjects of the Russian Federation.
Keywords: hemorrhagic fever with renal syndrome, incidence rates among the population, prophylaxis, deratization.
AUTHORS
Matrosov Aleksandr Nikolaevich – Doctor of Biological Sciences, Lead Researcher, Department of Epidemiology of Russian Research Anti-Plague Institute "Microbe"; Tel.: (8452) 26-21-31, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID: 0000-0003-4893-7188
Kuznetsov Aleksandr Nikolaevich – Doctor of Biological Sciences, Chief Researcher, Department of Epidemiology of Russian Research Anti-Plague Institute "Microbe". ORCID ID: 0000-0002-0677-4846
Ivanova Aleksandra Vasilevna – Candidate of Medical Sciences, Senior Researcher of Laboratory of Sanitary Protection and Emergency Situations of the Epidemiology Department of Russian Research Anti-Plague Institute "Microbe". ORCID ID: 0000-0002-4849-3866
Ermakov Nikolay Mikhaylovich – Candidate of Biological Sciences, Associate Researcher, Epidemiology Department of Russian Research Anti-Plague Institute "Microbe". ORCID ID: 0000-0001-9105-7448
Chumachkova Elena Arnoldovna – Researcher, Epidemiology Department of Russian Research Anti-Plague Institute "Microbe". ORCID ID: 0000-0001-9877-5258
Tolokonnikova Svetlana Ivanovna – Candidate of Biological Sciences, Senior Researcher, Epidemiology Department of Russian Research Anti-Plague Institute "Microbe". ORCID ID: 0000-0002-2070-0456
Kresova Ulyana Alekseevna – Candidate of Medical Sciences, Head of Epidemiological Laboratory of Center of Hygiene and Epidemiology in the Saratov Region
Tarasov Mikhail Alekseevich – Doctor of Biological Sciences, Zoologist of Center of Hygiene and Epidemiology in the Saratov Region. ORCID ID: 0000-0003-4615-8279
Romanov Roman Alekseevich – Zoologist of Center of Hygiene and Epidemiology in the Saratov Region
Sonin Vadim Konstantinovich – Zoologist of Center of Hygiene and Epidemiology in the Saratov Region
Dzyabko Alla Nikolaevna – Head of Disinfection Department of Center of Hygiene and Epidemiology in the Saratov Region
Matveeva Nadezhda Igorevna – Deputy Head of Rospotrebnadzor Department in the Saratov region
Arkhipova Galina Nikolaevna – Head of the Department of Epidemiological Supervision and Sanitary Protection of the Territory of Rospotrebnadzor Department in the Saratov region
Vyatkin Igor Nikolaevich – Deputy Head of the Department of Epidemiological Supervision and Sanitary Protection of the Territory of Rospotrebnadzor Department in the Saratov region
Popov Nikolay Vladimirovich – Doctor of Biological Sciences, Chief Researcher, Department of Epidemiology of Russian Research Anti-Plague Institute "Microbe". ORCID ID: 0000-0003-4099-9261
Kozhanova Olga Ivanovna – Head of Rospotrebnadzor Department in the Saratov region
OUR CONGRATULATIONS
p. 71
N.I. Schaschina (to her 70th anniversary)