Dezdelo prodaja 

disinf-want
 
 
Disinfection affairs № 3 / 2019


CONTENTS


Life of National Organization of Disinfectionists (NOD)

p. 5
Congratulation new members of NOD

 

DISINFECTION

pp. 5-15

DOI: 10.35411/2076-457X-2019-3-5-15

Comparative evaluation of the potentiating effect of nanoparticles of noble metals in the antimicrobial compositions

K.I. Gurin1, I.P. Pogorelsky1, G.A. Frolov2, V.S. Menukhova1, A.V. Mironin1, I.A. Lundovskikh3, V.S. Lobastov1, А.М. Shoev3

1Branch of the Federal State Budgetary Establishment "48 Central Scientific Research Institute" of the Ministry of Defense of the Russian Federation: 121 Oktyabrsky Ave, Kirov, 610017, Russian Federation;

2National University of Science and Technology "Moscow institute of steel and alloys": 4 Leninsky Ave., Moscow, 119991, Russian Federation;

3Vyatka State University: 36 Moskovskaya str., Kirov, 610000, Russian Federation.

The results of a comparative study of biocidal activity of stable aqueous colloidal solutions of silver, gold and platinum nanoparticles, Benzalkonium chloride antiseptic and their combinations in antimicrobial compositions in relation to the test microorganisms Escherichia coli C 600, Yersinia pestis EV line of the Research Institute of Epidemiology and Hygiene, Staphylococcus aureus KM, Bacillus cereus АТСС 10702 and Bacillus anthracis STI-1 spores are presented. The stability of aqueous colloidal solutions of silver, gold, and platinum nanoparticles is a consequence of the high value of the zeta potential (ζ-potential) exceeding -18,7 mV. The potentiating effect of silver, gold, and platinum nanoparticles of varying intensity has been revealed, resulting in an increase in the bactericidal and sporicidal activity of antimicrobial compositions based on the antiseptic Benzalkonium chloride containing aqueous colloidal systems in the series of platinum, gold, and silver nanoparticles. Based on the experimental data, an assumption was formulated that the equilibrium energy state of dispersed systems is achieved by compensating the energy transferred from high-energy surfaces of metal nanoparticles to water dipoles forming part of hydrated shells, resulting in an additional induced dipole moment leading to pronounced polarization of water molecules. Under these conditions, nanoparticles act as biocatalysts for intracellular enzymes, which cause the hydrolysis of polymeric structures of cytoplasmic membranes and cell walls and, as a result, the death of microbial cells.
Keywords: microorganisms, biocidal activity, antiseptic, nanoparticles, zeta potential.

Authors
Gurin Konstantin Igorevich – Candidate of Medical Sciences, Deputy Chief of Research Center of 48 Central Scientific Research Institute; служеб. тел.: (8332) 64-18-13, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.
Pogorelskiy Ivan Petrovich – Doctor of Medical Sciences, Professor, Leading Researcher of Research Center of 48 Central Scientific Research Institute. ORCID ID 0000-0001-6293-7366
Frolov Georgiy Aleksandrovich – Candidate of Chemistry Sciences, Associate Professor of Physical Chemistry of Moscow institute of steel and alloys. ORCID ID 0000-0003-1460-6030
Menukhova Valentina Sergeevna – Software Engineer of Branch of 48 Central Scientific Research Institute
Mironin Aleksandr Viktorovich – Doctor of Medical Sciences, Professor, Leading Researcher of Research Center of 48 Central Scientific Research Institute. ORCID ID 0000-0002-2434-2385
Lundovskikh Irina Aleksandrovna – Candidate of Chemistry Sciences, Associate Professor of Department of Microbiology of Vyatka State University. ORCID ID 0000-0003-4954-8870
Lobastov Vladimir Sergeevich – Candidate of Technical Sciences, Senior Research Associate of Research Center of 48 Central Scientific Research Institute
Shoev Aleksandr Maksimovich – Bachelor of Department of Microbiology of Vyatka State University

 

pp. 16-24

DOI: 10.35411/2076-457X-2019-3-16-24

Use of skin antiseptics based on alcohols (content analysis)

O.A. Melnikova

Federal State Budgetary Educational Institution of Higher Education "Urals State Medical University" of the Ministry of Healthcare of the Russian Federation:3 Repina str., Ekaterinburg, 620028, Russian Federation.

The study of skin antiseptics was analyzed. Used data from well-known libraries Cochrane Library, MEDLINE. The results of search filters are systematized into a table of primary data with an indication of the composition of the skin antiseptic, its effect on a certain group of microorganisms, the conclusions made by the authors of studies on the equivalence of skin antiseptics. The studies conducted in this direction are described in detail and a summary table of results is given, which shows that using 60 % isopropyl alcohol to disinfect the skin for 60 seconds gives a sufficient level of bacterial contamination reduction and is equivalent to using 70–75 % ethanol. The data of the European recommendations on the use of 60 % isopropyl alcohol as a reference solution in the evaluation of the antiseptic and disinfecting properties are given.
Keywords: аntiseptic, ethyl alcohol, isopropyl alcohol, skin.

Authors
Melnikova Olga Aleksandrovna – Doctor of Pharmaceutical Sciences, Professor of the Department of Management and Economics of Pharmacy of FSBEI НE USMU MOH Russia; Tel.: (343) 214-86-71, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID 0000-0002-1317-3109


DISINSECTION

pp. 25-40
DOI: 10.35411/2076-457X-2019-3-25-40

Characteristic of efficiency and toxicity of modern insectoacaricides, larvicides, insect growth regulators, and repellents. Part 2. Insecticides of plant origin, pyrethroids

S.A. Roslavtseva, M.V. Bidevkina, A.I. Vinogradova

Scientific Research Disinfectology Institute of the Federal Service for surveillance on consumer rights protection and human wellbeing: 18 Nauchny proezd, Moscow, 117246, Russian Federation.

Entomological and acarological characteristics for insectoacaricides of vegetable origin (rotenone, pyrethrins, neem oil, or azadirachtin) and pyrethroids (volatile: allethrin and its isomers prallethrin, transfluthrin, empenthrin, and non-volatile: containing cyano-group (cypermethrin, alpha-cypermethrin, cyphenothrin, cyhalothrin, etc.) and non-containing cyano-group (tetramethrin, permethrin, etc.) are given. Data on toxicity and hazard of these insectoacaricides including acute toxicity at intragastric, percutaneous, and inhalation ways of entering into organism, on irritant, skin resorptive, and sensibilizing action, presence of distant effects, and also hygienic standards in various environmental objects are presented. The historical reference of the synthesis and application of these insectoacaricides is given.
Keywords: insectitсides оf vegetable origin, pyrethroids, effectivity, toxicity, disinsection.

Authors
Roslavtseva Svetlana Aleksandrovna – Doctor of Science in Biology, Professor; Head of Scientific Bacisc and Deratization Department of Scientific Research Disinfectology Institute; tel.: (495) 332-01-76, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript..
Orcid ID 0000-0003-1684-2029
Bidevkina Marina Vasil’evna – Doctor of Medical Science, Head of Laboratory Concerning Toxicology in Scientific Research Disinfectology Institute. Orcid ID 0000-0001-6433-899X
Vinogradova Arina Igorevna – junior researcher of Laboratory Concerning Toxicology in Scientific Research Disinfectology Institute Orcid ID 0000-0002-3253-4571

 

pp. 40-46

DOI: 10.35411/2076-457X-2019-3-40-46

Determination of flea susceptibility to modern insecticides

Yu.M. Tokhov1, O.Yu. Eremina2, Yu.S. Artyushina1, E.A. Grishina3

1The Federal Government Health Institution "Stavropol Plague Control Researsh Institute" of the Federal Service for surveillance on consumer rights protection and human wellbeing: 13-15 Sovietskaya str., Stavropol, 355035, Russian Federation;

2Scientific Research Disinfectology Institute of the Federal Service for surveillance on consumer rights protection and human wellbeing: 18 Nauchny proezd, Moscow, 117246, Russian Federation;

3Ltd company "Laboratory of MediLis": 129 b. 2 Varshavskoe shosse, Moscow, 117587, Russian Federation.

The data on the susceptibility of flea laboratory cultures to modern insecticides containing fenthion, malathion, chlorpyrifos and imidacloprid are presented. The sensitivity of flea cultures of Citellophilus tesquorum and Nosopsyllus laeviceps to fenthion is 4–7 times more, to chlorpyrifos – 15 times more, to malathion – 1.8 times more than rat flea culture Xenopsylla cheopis. The culture of rat fleas was more heterogeneous in sensitivity to organophosphorus compounds in comparison with C. tesquorum and N. laeviceps. The results obtained are of interest to specialists in the field of disinsection, carrying out sanitary and anti-epidemic measures in the natural foci of the plague.
Keywords: natural focus, fleas, plague, insecticides, Citellophilus tesquorum, Nosopsyllus laeviceps, Xenopsylla cheopis.

Authors
Tokhov Yuriy Mukhamedovich – Doctor of Science in Biology, Leading Researcher of Laboratory of Medical Parasitology of Stavropol Plague Control Researsh Institute; Tel.: (8652) 26-18-19, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID 0000-0002-0562-6040
Eremina Olga Yurevna – Doctor of Science in Biology; Leading Researcher of Laboratory of Problems of Disinsection of "Scientific Research Disinfectology Institute" (SRDI); Tel.: (495) 332-01-51, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. Orcid ID 0000-0002-8268-2230
Artyushina Yuliya Sergeevna – Biologist of Laboratory of Problems of Disinsection of Stavropol Plague Control Researsh Institute. ORCID ID 0000-0002-7183-8260
Grishina Elena Aleksandrovna – Candidate of Medical Sciences, CEO of Ltd company "Laboratory of MediLis"

 

pp. 46-53

DOI: 10.35411/2076-457X-2019-3-46-53

Practical experience control of the tropical bed bug, Cimex hemipterus F., at the facilities of the city of Moscow and the Moscow region

R.A. Khryapin, V.Yu. Kapustin, A.A. Matveev

Test laboratory centre of the Moscow city centre of disinfection:9 Yaroslavskoe shosse, Moscow, 129337, Russian Federation

Occupancy of bed bugs in many large cities of the Russian Federation is currently a definite problem for organizations offering pest control services. Often, working with disinfectants that are time-approved to work on bedbugs, even experienced and responsible is infection officers do not get a positive effect either after the second or after the third disinsection treatment. Such situations lead not only to material costs, but also to a loss of reputation, which is especially sensitive for organizations with many years of experience, punctual performance of their duties, accumulated client bases. The article summarizes the practical experience of control the tropical bed bug, Cimex hemipterus F., at the facilities of the city of Moscow and the Moscow region. Resistance to the active substances and modern insecticidal formulations on their basis has been identified. Ways to overcome the resistance of this bed bugspecies to insecticides and the features of disinsection treatment in order to achieve the best results are considered. The most common causes of unsatisfactory results of insecticidal treatments carried out in order to eliminate bed bugs are analyzed. The main, based on their own experience, practical recommendations for the effective destruction of bed bugs in rooms are formulated.
Keywords: Cimex hemipterus, Cimex lectularius, bed bug control, insecticide resistance, medical pest control.

Authors
Khryapin Roman Aleksandrovich – Biologist of Laboratory of Biocontrol of Test laboratory centre of the Moscow city centre of disinfection (TLC MCCD); Tel.: (910) 440-87-51, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID 0000-0002-6448-5057
Kapustin Vladimir Yurevich – Head of Department of Quality Control of TLC MCCD. ORCID ID 0000-0002-0955-2341
Matveev Aleksandr Aleksandrovich – Biologist of Laboratory of Biocontrol of TLC MCCD. ORCID ID 0000-0002-5978-6314


DERATIZATION
pp. 54-59
DOI: 10.35411/2076-457X-2019-3-54-59

Using the method of reversed-phase variant of high performance liquid chromatography for the assessment of substances in the composition of foam for deratization

A.N. Kochetov1,2, А.А. Matveev1, L.A. Nosikova2,3

1Testing laboratory center of "Moscow city center of disinfection": 9 Yaroslavl highway, Moscow, 129337, Russian Federation;

2MIREA – Russian Technological University (M.V. Lomonosov Institute of Fine Chemical Technologies): 86 prospect Vernadskogo, Moscow, 119571, Russian Federation;

3A.N. Frumkin Institute of Physical Chemistry and Electrochemistry of the Russian Academy of Sciences (IPCE RAS): 31 Leninsky prospect, Moscow, 119071, Russian Federation.

Considers the possibility of analytical determination of substances 4-hydroxycoumarins range of the product appears and brodifacoum comprising a foam. Rodenticide foams are individual formulations that have recently occupied a unique niche, the use of which significantly expand the opportunities for the implementation of activities within the framework of "pest conrol". The optimal conditions for sample preparation were determined. The possibility of joint determination of substances by optimizing the conditions of division is shown. The reversed-phase variant of high performance liquid chromatography (RP HPLC) in isocratic mode (UV-detection) at 280 nm was used with the involvement of trivial analytical equipment. Sample preparation is reduced to solubilization of the preparative form canopies in an acidified mixture of isopropyl alcohol and water. As an eluent for chromatography, it is optimal to use a mixture of acetonitrile: water: acetic acid in a ratio of 70 : 30 : 1, while joint determination of substances is possible. Greater performance can be achieved by using more polar chromatography conditions. Used methodological approaches can enforce control on all production stages: input control of the technical sub-stations technology of premix – ready preparative forms foamy. Кeywords: rodenticides, foam rodenticides, bromadiolone, brodifacoum, RP HPLC, production control.

Authors
Alexander N. Kochetov – Head of the Chemical Laboratory of the Testing Laboratory Center of "Moscow City Center of Disinfection"; Tel.: (903) 562-15-89, E-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID 0000-0002-1911-1718
Alexander A. Matveev – Head of the Laboratory of Biocontrol of the Testing Laboratory Center of "Moscow City Center of Disinfection". ORCID ID 0000-0002-5978-6314
Lyubov A. Nosikova – Associate Professor of Chemistry and Technology of Rare and Scattered Elements and Nanoscale Composite Materials of the MIREA – Russian Technological University (Institute of Fine Chemical Technologies); Senior Scientist et al. Laboratory of New Physical and Chemical Problems of Institute of Physical Chemistry and Electrochemistry. A.N. Frumkin (IFHA) Wounds. ORCID ID 0000-0002-4144-5343

 

MICROBIOLOGY

pp. 60-68
DOI: 10.35411/2076-457X-2019-3-60-68

The collection of microorganisms cultures at the Scientic Research Disinfectology Institute

A.V. Ilyakova, N.V. Shestopalov, L.S. Fedorova

Scientific Research Disinfectology Institute of the Federal Service for surveillance on consumer rights protection and human wellbeing: 18 Nauchny proezd, Moscow, 117246, Russian Federation.

The article describes the basic principles of operation and features of conducting the collection of microorganisms cultures at the Scientific Research Disinfectology Institute. It provides information on the status of the collection at the moment, its species composition and structure. Disclosed culturing issues, storage, and determining a standard resistance reference crops to basic chemical disinfectants to be used to ensure the quality of research and testing disinfectants to evaluate their effectiveness.
Keywords: collections of microorganisms, strains of microorganisms, disinfectants, storage of microorganisms.

Authors
Ilyakova Anastasia Vasilievna – Junior Researcher of Laboratory of Disinfectology Problems of Scientific Research Disinfectology lnstitute (SRDI); Tel.: (495) 332-01-06, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID: 0000-0002-1867-3495
Shestopalov Nikolay Vladimirovich – Doctor of Medical Science, Professor; Director of (SRDI). ORCID ID: 0000-0002-9973-3508
Fedorova Lyudmila Samuilovna – Doctor of Medical Science, Professor; Head of Laboratory of Disinfectology Problems of (SRDI). ORCID ID: 0000-0002-3345-2631

 

SHARING EXPERIENCES

pp. 69-74
DOI: 10.35411/2076-457X-2019-3-69-74

On the issue of evaluation of safety and efficacy of disinfectants In their examination for the purpose of state registration in the Republic of Belarus

V.M. Vasilkevich1, R.V. Bogdanov1, O.V. Savinova2

1Republican Unitary Enterprise "Scientific and Practical Centre of Hygiene": 8 Academicheskaya str., Minsk, 220012, Republic of Belarus;

2State institution "Republican Scientific and Practical Center for Epidemiology and Microbiology": 23 Filimonova str., Minsk, 220114, Republic of Belarus.

The authors provide an overview and comparison of the main approaches to the safety assessment and registration of disinfectants (biocides) in the Republic of Belarus (within the framework of national and Eurasian legislation) and the European Union. Based on the material presented in the article and its analysis, an opinion was expressed on the main directions of improving and harmonizing domestic and European legislation in the field of safety assessment and registration of disinfectants.
Keywords: disinfectants, biocides, registration, disinfectological examination, chemical safety, improvement of legislation.

Authors
Vasilkevich Vadim Mikhaylovich – Candidate of Medical Science, Research Associate of Laboratory of Industrial Toxicology of Scientific and Practical Centre of Hygiene; Tel.: +375 (17) 284-13-91, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID 0000-0002-6461-0655
Bogdanov Ruslan Valerevich – Candidate of Medical Science, Head of Laboratory of Industrial Toxicology of Scientific and Practical Centre of Hygiene. ORCID ID 0000-0003-3655-4155
Savinova Olga Vladimirovna – Candidate of Medical Science, Research Associate of Republican Scientific and Practical Center for Epidemiology and Microbiology, Secretary of the Commission on Means of Disinfection of the Ministry of Health of Republic of Belarus. ORCID ID 0000-0002-1835-7724

OUR CONGRATULATIONS

p. 75
L.N. Mazin (to his 70th anniversary)

 

OBITUARY

p. 76

Kudryashov A.P. (1932-2019)