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Disinfection affairs № 2 / 2021

DISINFECTION

pp. 5-10
DOI: 10.35411/2076-457X-2021-2-5-10

Determination of quaternary ammonium salts and polyhexamethylene biguanide hydrochloride in the coexistence in disinfectants and their biocidal activity

Yu.V. Matveychuk, D.V. Stanishevskiy, Yu.V. Shabeka

LLC «NORDKHIM»: 23A/309, Uruchskaya str., Minsk, 220125, Republic of Belarus.

Titrimetric methods for determining the concentration of quaternary ammonium salts and polyhexame­thylenebiguanide hydrochloride in the presence of a disinfectant are described. In addition, as auxiliary components, the agent contains a nonionic surfactant and an alkyl polyglucoside and a disodium salt of ethylenediaminetetraacetic acid as a complexing agent.
The main differences between the methods proposed by us for the determination of quaternary ammonium salts and polyhexamethylenebiguanide hydrochloride is the use of not sodium dodecyl sulfate as a titrant, but a solution of the disinfectant itself, which greatly facilitated the visual fixation of the equivalence point.
In addition, the volume of the indicator (methylene blue and bromophenol blue) was selected in such a way that the exposure between the portions of the added titrant was not prolonged, because this would increase the overall duration of the analysis. When titrating with polyhexamethylenebiguanide hydrochloride, the vo­lume of the indicator is selected in such a way as to minimize the interfering effect of HR, which are also part of disinfectants. The reproducibility of the developed methods has been confirmed in accredited laboratories of the relevant state institutions of the Republic of Belarus.
Metrological characteristics of the developed methods: accuracy index±3.7 %, reproducibility index 4.0 %, n = 17, P = 0.95 – to determine the QAS; accuracy index±4.2 %, reproducibility index 5.5 %, n = 17, P = 0.95 – for the determination of PHMBG.
Keywords: disinfection, quaternary ammonium salts, polyhexamethylenebiguanid hydrochloride, titri­metry.

Authors
Matveichuk Yuliya Vladimirovna – PhD in Chemistry, Associate Professor, Leading Researcher, LLC «NORDKHIM»; Tel.: + 375 (29) 549-14-50, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.
Dmitrii Vladislavovich Stanishevsky – chemist of LLC «NORDKHIM»
Shabeka Yurii Viktorovich – Director of LLC «NORDKHIM»

pp. 10-15
DOI: 10.35411/2076-457X-2021-2-10-15

Assessment of the safety of skin antiseptics by the method of in vitro

M.V. Bidevkina, T.N. Potapova, M.V. Matrosenko

Scientific Research Disinfectology Institute of Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing: 18 Nauchny proezd, Moscow, 117246, Russian Federation.

InVitro laboratory has investigated and made comparative analysis of irritant effect of disinfectants on rabbits. Main active substances of studied skin antiseptics were: alcohols, chlorhexidine bigluconate, alkyldime­thylbenzylammonium chloride, didecyldimethylammonium chloride, N,N-bis(3-aminopropyl)dodecylamine. Has been developed method for evaluating the irritant effect of skin antiseptics on bull sperm suspensions.
Keywords: disinfectants, skin antiseptics, irritant effect, rabbits, bull spermatozoa.

Authors
Bidevkina Marina Vasilevna – Doctor of Medical Science, Leading Researcher – Head of Laboratory Concerning Toxicology in Scientific Research Disinfectology Institute; Tel.: (495) 332-01-55, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID: 0000-0001-6433-899X
Potapova Tatyana Nikolaevna – Senior Researcherof Laboratory Concerning Toxicology in Scientific Research Disinfectology Institute. ORCID ID: 0000-0003-1089-7037.
Matrosenko Margarita Vladimirovna – Junior Researcher of Laboratory Concerning Toxicology in Scientific Research Disinfectology Institute. ORCID ID: 0000-0003-0567-2247

DISINSECTION

pp. 15-22
DOI: 10.35411/2076-457X-2021-2-15-22

The study of neonicotinoids baits on susceptible laboratory culture of pharaoh ants

O.Yu. Eremina, V.V. Olifer

Scientific Research Disinfectology Institute of Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing: 18 Nauchny proezd, Moscow, 117246, Russian Federation.

Comparative efficacy of neonicotinoid-based baits (thiamethoxam, imidacloprid, acetamiprid) intended for control of the Pharaoh ant was studied. The death of workers, queens and brood occurs within 2–4 weeks. The minimum effective concentrations of thiamethoxam and imidacloprid in granulated sugar, which ensure the death of ant colonies in the presence and absence of an alternative food, were revealed. Under laboratory conditions, when the colonies of the Pharaoh ant were fed with such baits, after 4 weeks of the experiment the depletion of the colony was noted (death of queens was 75–100 %, and critical decrease in the number of live workers and brood was occured). Dry sugar baits based on 0.1–1.0 % acetamiprid have no significant effect on the viability of the ant colony.
Keywords: Pharaoh ant, baits, thiamethoxam, imidacloprid, acetamiprid.

Authors
Eremina Olga Yurevna – Doctor of Science in Biology; Leading Researcher of Laboratory of Problems of Disinsection of Scientific Research Disinfectology Institute; Tel.: (495) 332-01-51, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID: 0000-0002-8268-2230
Olifer Veronika Valentinovna – Candidate of Science in Biology; Leading Researcher of Laboratory of Problems of Disinsection of Scientific Research Disinfectology Institute. ORCID ID: 0000-0002-4736-7905

pp. 22-29
DOI: 10.35411/2076-457X-2021-2-22-29

The use of model objects in evaluating the effectiveness of DEET-based repellent agents for individual protection against blood-sucking arthropods

O.M. Germant, N.I. Shashina

Scientific Research Disinfectology Institute of Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing: 18 Nauchny proezd, Moscow, 117246, Russian Federation.

With the aim of unifying and simplifying the assessment of categories of the effectiveness of the repellents funds audited 473 studies 245 samples of repellent remedies on the basis of DEET in various formulations, made in 1995–2019 employees of the Scientific Research Disinfесtology Institute in laboratory and in field conditions. The experiments were conducted in parallel with the standards of the corresponding or expected categories of effectiveness of repellent remedies. The concordance of the results of standard laboratory experiments on a model object Ae. aegypti mosquitoes and the results obtained from testing repellent agents against natural populations of flying blood-sucking insects and taiga ticks is proved. The effectiveness of repellent remedies based on other repellent substances, as well as the effectiveness against other species, should continue to be investigated in full-scale conditions for individuals of natural populations. These remedies should be included in the system of categories of effectiveness of repellent products.
Keywords: model objects, repellent remedies, DEET, mosquitoes, blood-sucking insects, ticks.

Authors
Germant Olga Mikhaylovna – Leading Researcher of Laboratory of Problems of Disinsection of Scientific Research Disinfectology Institute; Tel.: (495) 332-01-81, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.
Shashina Natalya Igorevna – Doctor of Science in Biology; Leading Researcher of Laboratory of Problems of Disinsection of Scientific Research Disinfectology Institute. ORCID ID: 0000-0003-2537-8926

pp. 30-36
DOI: 10.35411/2076-457X-2021-2-30-36

Reversibility of the knockdown effect at application of aerosols against pyrethroid-resistant house flies

T.A. Davlianidze

Scientific Research Disinfectology Institute of Federal Service in Supervision of Protection of Rights of Consumers and Prosperity of a Man: 18 Nauchny proezd, Moscow, 117246, Russian Federation.

Knockdown resistance to pyrethroids and pyrethrins is the first sign of insecticide resistance, suggesting a decrease in insect nervous system sensitivity. On the Russian market of insecticides, there are many products in the aerosol package intended for the destruction of flying insects, which include pyrethroids, which provide a quick knockdown effect in insecticide susceptible insect strains. In resistant populations, in many cases, the reversibility of paralysis is observed, which indicates insufficient effectiveness of insecticidal products. The insecticidal activity of four products in aerosol package containing pyrethroids or pyrethrins in various concentrations on houseflies in natural populations was studied in comparison with the insecticide susceptible against laboratory on strain S-NIID. The most effective formulation was on the basis of natural pyrethrins. The reversibility of paralysis of resistant populations was detected using all aerosols and amounted to 0–37 % for the Kaluga strain, 10–93 % for the Krasnogorsk, KSK-1 0–87 %, KSK-2 20–99 %, S-NIID 0 %.
Keywords: resistance, knockdown effect, pyrethroids, paralysis reversibility, insecticides, housefly.

Authors
Davlianidze Tatyana Alekseevna – Laboratory Research Assistant of Laboratory of Problems of Disinsection of SRDI; Tel.: (495) 332-01-51, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID: 0000-0002-2757-881X

pp. 36-43
DOI: 10.35411/2076-457X-2021-2-36-43

New data on distribution of invasive mosquito species in the Europe and in Russia

S.A. Roslavtseva, A.I. Frolova, M.A. Alekseev

Scientific Research Disinfectology Institute of Federal Service in Supervision of Protection of Rights of Consumers and Prosperity of a Man: 18 Nauchny proezd, Moscow, 117246, Russian Federation.

Literature on distribution of invasive mosquito species that are vectors of especially dangerous infections (dengue, Chikungunya, Zika, yellow fever, and Japanese encephalitis) in the Europe and Russia was briefly reviewed. Global warming of the climate as well as an increase in the volume of world trade and the biological characteristics of mosquitoes contribute to the widespread of these species on territories of many countries and continents
Keywords: mosquitoes, Aedes albopictus, Aedes aegypti, Aedes koreicus, Aedes japonicus japonicus, Aedes atropalpus, arboviral fevers, Japanese encephalitis.

Authors
Roslavtseva Svetala Aleksandrovna – Doctor of Science in Biology, Professor; Head of Scientific Bacisc and Deratization Department of Scientific Research Disinfectology Institute; tel.: (495) 332-01-76, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID: 0000-0003-1684-2029
Frolova Alla Iosifovna –  Candidat of Biological Sciences, Leading Researcher of Scientific Bacisc and Deratization Department of Scientific Research Disinfectology Institute. ORCID ID: 0000-0003-4350-6303
Alekseev Mikhail Anatol’evich – Candidat of Biological Sciences, Head of Insectary, Senior Research Associate of Scientific Bacisc and Deratization Department of Scientific Research Disinfectology Institute. ORCID ID: 0000-0001-7562-1782

EPIDEMIOLOGY

pp. 44-50
DOI: 10.35411/2076-457X-2021-2-44-50

Features of the dynamics of hemorrhagic fever with renal syndrome incidence in the Republic of Bashkortostan

P.A. Mochalkin1, A.P. Mochalkin1, E.G. Stepanov2, L.A. Farvazova2, N.V. Popov3, S.N. Nemtsova4

1State Budgetary Health Institution «Republican Center of Disinfection», Ministry of Health of the Republic of Bashkortostan: 127/1 Mingazheva St., Ufa City, Republic of Bashkortostan, 450005, Russian Federation;
2Rospotrebnadzor Administration in the Republic of Bashkortostan: 58 R. Zorge St., Ufa City, Republic of Bashkortostan, 450054, Russian Federation;
3Federal Government Health Institution «Russian Research Anti-Plague Institute «Microbe» of the Rospotrebnadzor: 46 Universitetskaya St., Saratov, 410005, Russian Federation;
4Ufa Medical and Preventive Company: 4 Tramvaynaya str., Ufa, Republic of Bashkortostan, 450027, Russian Federation.

It was established that 17008 HFRS cases were reported in 53 (out of 54) administrative districts and 12 cities (out of 14) of the Republic of Bashkortostan in 2009-2018. The most intense epidemiological situation (a long-term incidence rate of 48,5 per 100 thousand of the population) was observed within the boundaries of the forest-steppe landscape zone, where. more than 86 % of all cases of infection are recorded of the Republic of Bashkortostan in 2009-2018. The share of infections among the urban population was 64.0 %, the rural one – 36.0 %. In the long-term aspect, according to the conditions of HFRS acquisition, short-term visits to the forest area prevailed – up to 80 %, in household settings – up to 14 % of all cases of the disease. Seasonal increases in the incidence of HFRS take place in May-June – up to 15 % and in September-December – over 46 % of the annual incidence. The presence of synchronicity in the dynamics of HFRS morbidity in rural and urban populations within the boundaries of the administrative district was detected. It has been substantiated that in order to reduce the level of HFRS morbidity among the rural and urban population, it is necessary to provide for an increase in the volume and frequency of preventive (anti-epidemic) measures in the vicinity of rural settlements, regional centers, urban-type settlements and large cities located near forests, within the boundaries of the entire area of the administrative district.
Keywords: hemorrhagic fever with renal syndrome, factors of risk of infection, urban and rural population, tactics of non-specific prophylaxis.

Authors
Mochalkin Pavel Alexandrovich – Candidate of Medical Sciences, Chief Medical Officer at the State Budgetary Health Institution «Republican Center of Disinfection», Ministry of Health of the Republic of Bashkortostan: Tel. (347) 273-83-27:, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.; ORCID ID: 0000-0001-7415-1299
Mochalkin Alexander Pavlovich
Stepanov Evgeny Georgievich – Candidate of Medical Sciences, Head of the Rospotrebnadzor Administration in the Republic of Bashkortostan, Chief State Sanitary Officer in the Republic of Bashkortostan
Farvazova Lira Amirovna – Deputy Head of the Department of the Rospotrebnadzor Administration in the Republic of Bashkortostan
Popov Nikolai Vladimirovich – Doctor of Biological Sciences, Professor, Head of the Laboratory of Epizootiological Monitoring at RusRAPI «Microbe». ORCID ID: 0000-0003-4099-9261
Nemtsova Svetlana Nikolaevna – General Director of Ufa Medical and Preventive Company

Life of National Organization of Disinfectionists (NOD)

Materials of scientific and practical conference "Current issues of disinfection
activities in the prevention of infections, including coronavirus infection" (May 19–20, 2021, St. Petersburg)

pp. 50-53

Reporting

pp. 53-58

N.A. Vatolina, Yu.M. Shishkin, I.G. Kakuchaya

Disinfection and quality control issues disinfection measures in epidemic centers at the present stage


pp. 58-62

L.I. Kabay, A.M. Datskevich, N.A. Vatolina

Distribution of bed bugs in the city St. Petersburg and the organization of the fight against them in modern conditions

pp. 62-65

I.G. Kakuchaya, Yu.M. Shishkin, N.A. Vatolina

Experience in organizing final disinfection in pandemic conditions a new coronavirus infection COVID-19


pp. 65-68

N.A. Vatolina, Yu.M. Shishkin, L.P. Alshanik, N.A. Pavlenko, A.A. Zenchenko

Study of antimicrobial activity of a mobile multifunctional station designed for cleaning and disinfection of surfaces in medical organizations


pp. 68-70

A.A. Zenchenko, N.A. Vatolina, Yu.M. Shishkin, L.P. Alshanik

Experience of using various personal protective equipment in the foci of a new coronavirus infection COVID-19


pp. 70-74

A.V. Domkin, G.N. Golukhov, N.M. Efremova, S.A. Tsykin, K.S. Safaryan

Organization of disinfection measures in conditions of a somatic hospital repurposed for work with COVID-19


pp. 74

Congratulation new members of NOD