DISINFECTION

pp. 5-9
DOI: 10.35411/2076-457X-2023-4-5-9

Effect of rheological properties on the antimicrobial activity of isopropyl alcohol

V.A. Novikov, F.A. Mukabenov, L.G. Panteleeva, N.I. Eremeeva, L.I. Istomina, S.V. Andreev

Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F.F. Erisman: 18a Nauchniy proezd, Moscow, 117246, Russian Federation.

Gels are one of the most comprehensive forms of hand hygiene antiseptics. Alcohol-based gels are used in many hospitals around the world for hand antisepsis. The main reason for using antiseptics in gel form is the reduction of skin irritation and dryness and the ease of use (no spillage as quickly as liquid antiseptics when tilting or tipping the bottle). However, gels take longer to dry than other forms, depending on the consistency of the gel. The change in consistency (rheology) has a significant effect on the rate of alcohol evaporation and therefore determines the contact time of the active ingredient with the skin. Since it is believed that the main contribution to antimicrobial activity is made by the active ingredient, and the other components are considered inert, in this work we tested model skin antiseptics based on isopropyl alcohol with different viscosity to evaluate the effectiveness of antiseptics in hand hygiene treatment was carried out in vivo on volunteers. The results obtained do not allow us to unambiguously interpret the effect of viscosity on antimicrobial activity, since up to a gel-former concentration of 1.5 % we observed a decrease in efficacy, and at a concentration of 2 % we observed a concentration higher than that for the aqueous solution of isopropyl alcohol. It follows from our findings that the issue of the influence of viscosity on the antimicrobial activity of skin antiseptics should be studied during disinfectological examination.
Keywords: skin antiseptics, gels, rheological properties, hospital-acquired infection.

Authors
Vyacheslav A. Novikov – Junior Researcher, Department of Disinfection and sterilization, Disinfectology Institute of F.F. Erisman FSCH of Rospotrebnadzor; Tel.: (495) 586-11-44; e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID: 0000-0001-8858-5872
Fedor A. Mukabenov – Researcher, Department of Disinfection and Sterilization, Disinfectology Institute of F.F. Erisman FSCH of Rospotrebnadzor. ORCID ID: 0000-0002-5631-0775
Liudmila G. Panteleeva – PhD in Medicine, Chief Expert, Disinfectology Institute of F.F. Erisman FSCH of Rospotrebnadzor. ORCID ID: D 0000-0002-9470-1750
Natalia I. Eremeeva – PhD in Biology, Head of Department of Disinfection and Sterilization, Disinfectology Institute of F.F. Erisman FSCH of Rospotrebnadzor. ORCID ID: 0000-0003-3637-2570
Liudmila I. Istomina – Junior Researcher, Department of Chemistry, Disinfectology Institute of F.F. Erisman FSCH of Rospotrebnadzor. ORCID ID: 0000-0003-1440-3778
Sergey V. Andreev – PhD in Chemistry, Head of Chemical Department, Disinfectology Institute of F.F. Erisman FSCH of Rospotrebnadzor ORCID ID: 0000-0003-2405-9931

pp. 10-20
DOI: 10.35411/2076-457X-2023-4-10-20

Comparative assessment of the toxicity and virucidal properties of disinfectants used when working with the SARS-CoV-2 virus in isolated virology laboratories

V.V. Zolin, O.P. Os’kina, V.V. Solodky, T.A. Gosteva, M.N. Eremina

Federal Budgetary Institution of Science "State Scientific Center of Virology and Biotechnology "Vector" of the Federal Service for Supervision in the Field of Consumer Rights Protection and Human Well-Being: Koltsovo village, Novosibirsk region, 630559, Russian Federation.

The choice of disinfectants and their combinations for the final disinfection of the premises of "infectious" areas of laboratories of biosafety level 3–4, in which work is carried out with viruses with epidemic potential, is important for reducing the toxic effect of these disinfectants on personnel while maintaining 100 % disinfection efficiency. Purpose of the study: to study the toxicity of disinfectants of different groups for cell culture, to select a toxicity neutralizer, to study the virucidal activity of these disinfectants and their combinations against the SARS-CoV-2 virus. Materials and methods: experimental studies were carried out using the reference strain of coronavirus SARS-CoV-2, namely the hCoV-19/Australia/VIC01/2020 (AUS) strain (Wuhan variant), obtained from the State Collection of Causative Agents of Viral Infectious Diseases and Rickettsial Diseases, functioning on the basis of the Federal Budgetary Institution State Scientific Center for Virology and Biotechnology "Vector" of Rospotrebnadzor. The residual infectious activity of the virus was determined by titrating samples in cell culture. To conduct the research, we used a Vero E6 cell culture obtained from the Collection of Cell Cultures of the Federal Budgetary Institution of Scientific Research Center for Virology and Biochemistry "Vector" of Rospotrebnadzor in the form of a 2-day monolayer with a confluency of 95–100 %. Results. As a result of the studies, experimental data were obtained on the dynamics of the virucidal activity of different groups of disinfectants and their combinations against the SARS-CoV-2 virus. The degree of toxicity of disinfectants for Vero E6 cell culture is shown. Optimal combinations of disinfectants and a 40 % aqueous solution of formaldehyde (hereinafter referred to as formalin) have been selected, increasing its disinfection efficiency at an initial concentration halved, which can be used for final disinfection treatment of the premises of isolated virology laboratories conducting work with the SARS-CoV- coronavirus. Conclusion. The results obtained indicate that when using selected combinations of disinfectants to carry out final disinfection with formaldehyde vapors formed during the evaporation of formaldehyde as a result of its heating, a twofold decrease in the initial concentration of formalin is possible, leading to a decrease in its toxic effect on personnel, while maintaining 100 % efficiency disinfection.
Keywords: disinfection, disinfectants, formaldehyde, SARS-CoV-2 coronavirus, disinfection efficiency, cytotoxicity.

Authors
Vladimir V. Zolin – Ph.D., Head of the Biosafety Department of the Federal State Scientific Center for Virology and Biotechnology "Vector" of Rospotrebnadzor; Tel.: (383) 363-47-10, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID: 0000-0003-4120-1178
Oksana P. Os’kina – Head of the Laboratory of the Biosafety Department of the Federal State Scientific Center for Virology and Biotechnology "Vector" of Rospotrebnadzor. ORCID ID: 0000-0002-9165-1524
Vladislav V. Solodky – Researcher at the Biosafety Department of the Federal State Budgetary Institution of the World Bank "Vector" of Rospotrebnadzor. ORCID ID: 0000-0002-7270-0028
Tatiana A. Gosteva – Junior Researcher of the Biosafety Department of the Federal State Budgetary Institution of the World Bank "Vector" of Rospotrebnadzor. ORCID ID: 0009-0004-1508-9623
Marina N. Eremina – Specialist of the Biosafety Department of the Federal State Budgetary Institution of the World Bank "Vector" of Rospotrebnadzor. ORCID ID: 0009-0008-5037-2706

DISINSECTION

pp. 20-26
DOI: 10.35411/2076-457X-2023-4-20-26

The comparative studying food bait for multi-resistant german cockroaches

O.Yu. Eremina, V.V. Olifer

Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F.F. Erisman: 18a Nauchniy proezd, Moscow, 117246, Russian Federation.

The effectiveness of six food baits containing hydramethylnon, imidacloprid, dinotefuran, acetamiprid against the German cockroach of four laboratory cultures – insecticide-susceptible S-NIID and multi-resistant MAG, CR and DM, was evaluated. A slowdown in the manifestation of symptoms of poisoning in cockroaches of resistant cultures was revealed in comparison with susceptible ones. LT95 resistance indices for bait based on hydramethylnon were 0.7–1.1, dinotefuran – 10->21, imidacloprid – 1.2–1.6, acetamiprid – 1.5–2.4. By day 21, in some variants of experiments, 5 % of males of resistant cultures and 5–45 % of females survived.
Keywords: German cockroach, resistance, food baits, resistance index. 

Authors
Eremina Olga Yurevna – Doctor of Science in Biology; Leading Researcher of the Division of disinsection (with laboratory of entomology) of the Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F.F. Erisman; Tel.: (495) 332-01-51, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID: 0000-0002-8268-2230
Olifer Veronika Valentinovna – Candidate of Science in Biology; Leading Researcher of the Division of disinsection (with laboratory of entomology) of the Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F.F. Erisman. ORCID ID: 0000-0002-4736-7905

pp. 27-33
DOI: 10.35411/2076-457X-2023-4-27-33

Use of entomopathogenic fungi against bed bugs

K.S. Krivonos, O.Yu. Eremina, V.V. Olifer

Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F.F. Erisman: 18a Nauchniy proezd, Moscow, 117246, Russian Federation.

A literature review on the effectiveness of the use of entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana for the destruction of bed bugs is given. Bed bug resistance to pyrethroids does not result in cross-resistance to infection with B. bassiana spores. Insect mortality is highly dependent on the concentration of fungal conidia, which is critical to induce horizontal transfer capable of increasing insect population mortality without the need for direct contact between bed bugs and the inoculated substrate. Pyrethroid residues on treated surfaces significantly reduce the germination of B. bassiana spores. At present, resistance of insects to mycoinsecticides in the field has not been revealed, however, it has been shown that in laboratory conditions its occurrence is possible.
Keywords: entomopathogenic fungi, Metarhizium, Beauveria, biopesticides, bed bug, resistance.

Authors
Krivonos Ksenia Sergeevna – PhD in Biological Sciences; Senior Researcher of the Division of disinsection (with laboratory of entomology) of the Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F.F. Erisman of Rospotrebnadzor; of the Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F.F.Erisman of Rospotrebnadzor; Tel.: (495) 332-01-56, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.; ORCID ID: 0000-0003-4633-6645
Eremina Olga Yurevna – Doctor of Science in Biology; Leading Researcher of the Division of disinsection (with laboratory of entomology) of the Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F.F. Erisman of Rospotrebnadzor; e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID: 0000-0002-8268-2230
Olifer Veronika Valentinovna – PhD in Biological Sciences; Leading Researcher of the Division of disinsection (with laboratory of entomology) of the Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F.F. Erisman of Rospotrebnadzor. e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.; ORCID ID: 0000-0002-4736-7905

EPIDEMIOLOGY

pp. 33-45
DOI: 10.35411/2076-457X-2023-4-33-45

Review of current epidemiological risks of introducing infectious diseases into the Russian Federation from the African continent

A.V. Ivanova, A.A. Zubova, Zh.A. Kas’jan, L.N. Dmitrieva, E.A. Chumachkova, A.A. Zimirova, S.A. Shcherbakova, V.V. Kutyrev

Russian Anti-Plague Institute "Microbe": 46 Universitetskaya str., Saratov, 410005, Russian Federation.

The paper presents generalized epidemiological data and analyzes the situation in the countries of the African region on infectious diseases requiring measures for sanitary protection of the territory of the Russian Federation. The epidemiological analysis was carried out on the basis of data from the official website of the African Bureau of the World Health Organization (WHO), the African Center for Disease Prevention and Control (CDC), Ministries of Health of the relevant countries, other international organizations, as well as materials of publications in the public domain. The paper summarizes and systematizes data on the incidence and territorial spread of diseases in the region from the perspective of possible risks for those visiting it. The presented data on infectious diseases make it possible to navigate issues related to the risk of infection with infectious diseases, to determine the factors and seasonality of the increased risk of infection, to predict the possibility of importing diseases to the Russian Federation.
Keywords: infectious diseases, WHO African region, ensuring sanitary and epidemiological well-being, risk of drift, sanitary protection of the territory, plague, cholera, malaria, EVD, MVD. 

Authors
Ivanova Aleksandra V. – Candidate of Medical Sciences, Senior Researcher of the Laboratory of Epidemiological Analysis and Forecasting of the Russian Research Anti-Plague Institute "Microbe"; Tel.: (8452) 73-46-48, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID: 0000-0002-4849-3866
Zubova Anastasiya A. – Junior Researcher of the Laboratory of Sanitary Protection and Emergency Situations of the Russian Research Anti-Plague Institute "Microbe". ORCID ID: 0000-0002-8741-5756
Kas’jan Zhanetta A. – Candidate of Medical Sciences, Senior Researcher of the Laboratory of Sanitary Protection and Emergency Situations of the Russian Research Anti-Plague Institute "Microbe". ORCID ID: 0000-0002-9828-3277
Dmitrieva Lyudmila N. – Researcher of the Laboratory of Sanitary Protection and Emergency Situations of the Russian Research Anti-Plague Institute "Microbe". ORCID ID: 0000-0002-3564-985X
Chumachkova Elena A. – Researcher of the Laboratory of Sanitary Protection and Emergency Situations of the Russian Research Anti-Plague Institute "Microbe". ORCID ID: 0000-0001-9877-5258
Zimirova Anastasiya A. – Junior Researcher, of the Laboratory of Sanitary Protection and Emergency Situations of the Russian Research Anti-Plague Institute "Microbe". ORCID ID: 0009-0004-5648-7003
Shcherbakova Svetlana A. – Doctor of Biological Sciences, Deputy Director for Scientific and Experimental Workof the Russian Research Anti-Plague Institute "Microbe". ORCID ID: 0000-0003-1143-4069
Kutyrev Vladimir V. – Academician of the Russian Academy of Sciences, Doctor of Medical Sciences, Professor; Director of the Russian Research Anti-Plague Institute "Microbe". ORCID ID: 0000-0003-3788-3452

Materials of scientific and practical conference and General meeting (32^th congress) of members of NOD (October 4–5, 2023, Moscow)

pp. 46-50

Report

pp. 51-55

The catalogue of participant of exhibition

DISINFECTION BUSINESS MANAGEMENT

pp. 56-59
DOI: 10.35411/2076-457X-2023-4-56-59

Strategy for the development of training in the field of medical and preventive affairs in St. Petersburg at the current stage

N.A. Vatolina^1,3, N.V. Fedotova¹, Yu.M. Shishkin², L.V. Lyalina^3,4, T.V. Osmirko⁴

¹SPb GBPEI "Medical College No. 3": 9 Educational per, St. Petersburg, 194354, Russian Federation;
²"St. Petersburg City Disinfection Station": 4b Finskiy per., St. Petersburg, 195009. Russian Federation;
³FBUN "St. Petersburg Research Institute of Epidemiology and Microbiology named after Pasteur" Rospotrebnadzor: 14 Mira st., St. Petersburg, 197101. Russian Federation;
⁴FGBOU VO "North-Western State Medical University named after I.I. Mechnikov": 41 Kirochnaya st., St. Petersburg, 191015, Russian Federation.

The development of the training system in the field of medical and preventive affairs in St. Petersburg was analyzed. The main tasks of staffing organizations and health care institutions, including the disinfection profile, are noted, which require immediate solution
Keywords: licensing of disinfection activities, sanitary and epidemiological service, organizations and institutions of disinfection profile, medical organizations, secondary vocational education in the specialty "medical and preventive business".

Authors
Vatolina Natalya Anatolyevna – Teacher of St. Petersburg State Budgetary Institution "Medical College No. 3", applicant for the degree of FBUN "St. Petersburg Research Institute of Epidemiology and Microbiology named after Pasteur," Tel.: (981) 944-61-33, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.
Fedotova Natalia Vitalievna – Director of St. Petersburg State Budgetary Institution "Medical College No. 3"
Shishkin Yuriy Mikhaylovich – chief physician of St. Petersburg State Budgetary Institution "St. Petersburg City Disinfection Station" (2019–2022)
Lyalina Lyudmila Vladimirovna – Doctor of Medical Sciences, Professor, Head of the Laboratory of Epidemiology of Infectious and Non-Communicable Diseases of the Federal Budgetary Institution "St. Petersburg Research Institute of Epidemiology and Microbiology named after Pasteur" Rospotrebnadzor
Osmirko Tatyana Vladimirovna – Candidate of Medical Sciences, Associate Professor of the Department of Epidemiology, Parasitology and Disinfectology, FSBEI HE "Northwestern State Medical University named after I.I. Mechnikov"

BEST PEOPLE AND ORGANISATIONS OF NOD

pp. 60-65

Orenburg disinfection station (100^th anniversary of its foundation)

V.M. Sherstnev

pp. 66-72

St. Petersburg Disinfection Station (140^th anniversary of its foundation)

N.A. Vatolina, V.Yu. Nazarov, Yu.M. Shishkin

Authors
Vatolina Natalya Anatolevna – Deputy Chief Physician for the Medical Department of St. Petersburg State Budgetary Institution "St. Petersburg City Disinfection Station" (2018-2022); Tel.: (981) 944-61-33, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.
Nazarov Vladimir Yurevich – Chief Physician of St. Petersburg State Budgetary Institution "St. Petersburg City Disinfection Station" (2005-2019)
Shishkin Yuriy Mikhaylovich – Chief Physician of St. Petersburg State Budgetary Institution "St. Petersburg City Disinfection Station" (2019-2022)

Life of National Organization of Disinfectionists (NOD)

p. 72

Congratulation new members of NOD

MEMORIALS

pp. 73-74

Shandala Mikhail Georgievich (to his 95^th anniversary)

pp. 74-75

Sokolova Natalya Filippovna (to her 100^th anniversary)