The effectiveness of the application of disinfectant using a hot fog generator
K.I. Gurin1, I.P. Pogorelsky1, M.A. Cheshuina1, V.S. Lobastov1, S.V. Zolotov1, A.V. Mironin1, A.V. Senkin1, I.A. Lundovskikhх2
1Scientific-Research Department of the Scientific-Research Centre of «48 Central Scientific Research Institute» of the Ministry of Defense of the Russian Federation: 119 Oktyabrsky Avenue, Kirov, 610000, Russian Federation;
2FSBEI HE «Vyatka State University»: 36 Moskovskaya str., Kirov, 610000, Russian Federation.
The sporicidal and bactericidal effect of hydrogen peroxide using a hot fog generator on the viability and ultrastructure of spores of the vaccine strain Bacillus anthracis STI-1 was studied using a bacteriological method and electron microscopy. The high efficiency of disinfection treatment of various surfaces has been established, which consists in the fact that within 10-20 minutes there is a complete death of test microorganism that contaminate the surfaces treated with hot fog. It has been experimentally proven that in the process of generating hot fog, the concentration of working disinfectant solution increases, which is based on the partial evaporation of moisture from solution of disinfectant during the formation of hot fog and its transfer by a stream of hot gas. Based on the results obtained, it is advisable to use hot fog generators to achieve a pronounced biocidal effect when carrying out disinfection measures in medical organizations, as well as when performing scientific research.
Keywords: microorganisms, spores, bacteria, hydrogen peroxide, hot fog generator.
Pogorelsky Ivan Petrovich – Doctor of Medical Sciences, Professor, Leading Researcher of the Scientific and Research Department
Cheshuina Marina Anatol’evna – Junior Researcher of the Scientific and Research Department.
Lobastov Vladimir Sergeevich – Candidate of Technical Sciences, Senior Researcher of the Scientific and Research Department
Zolotov Sergey Valer’evich – Candidate of Technical Sciences, Researcher of the Scientific and Research Department
Mironin Alexandr Viktorovich – Doctor of Medical Sciences, Professor, Leading Researcher of the Scientific and Research Department
Senkin Alexandr Vladimirovich – Candidate of Medical Sciences, Junior Researcher of the Scientific and Research Department
Lundovskikh Irina Aleksandrovna – Candidate of Chemistry Sciences, Associate Professor of Department of Microbiology of Vyatka State University. ORCID ID: 0000-0003-4954-8870
Investigation of the decontaminating activity of different disinfectant classes against nucleic acids
V.V. Kuzin, N.V. Kolupaeva, E.S. Kuzina, V.D. Potapov
State Research Center of Applied Microbiology and Biotechnology of Rospotrebnadzor: Territory «Kvartal A», Obolensk, Moscow region, 142279, Russian Federation.
Contamination of laboratory surfaces with nucleic acids and their amplicons is one of the most important problems encountered in nucleic acid amplification methods due to the occurrence of unreliable results. The aim of this study was to select and determine effective regimens for the use of various active agents for decontamination in PCR laboratories. The ability of ethyl alcohol, alkyldimethylbenzylammonium chloride, N,N-bis(3-aminopropyl)dodecylamine, polyhexamethyleneguanidine hydrochloride, hydrogen peroxide, peracetic acid, chlorine dioxide, sodium hypochlorite and neutral anolyte to destroy or irreversibly modify DNA, preventing its subsequent amplification was studied. The decontamination activity was analyzed by simulating the surface contamination with both long (1500 bp) and short (94 bp) amplicons. Hydrogen peroxide 2 %, peracetic acid 0.24 %, dichloroisocyanuric acid 0.01 %, sodium hypochlorite 0.1 % and chlorine dioxide 0.01 % were shown to have decontaminating ability. Notably, dichloroisocyanuric acid decontaminated surfaces from DNA at a concentration 20 times lower than previously described, and sodium hypochlorite at half the concentration, and chlorine dioxide was also found to have decontaminating activity. The absence of decontaminating activity was observed in ethyl alcohol 70 %, alkyldimethylbenzylammonium chloride 2 %, N,N-bis(3-aminopropyl)dodecylamine 2 %, polyhexamethyleneguanidine hydrochloride 2 % and neutral anolyte 0,05 %. The results obtained allow expanding the list of disinfectants recommended for decontamination measures in laboratories using nucleic acid amplification methods in order to prevent contamination of nucleic acids and their amplicons.
Keywords: PCR, DNA, amplicons, decontamination, disinfectants.
Kolupaeva Nadezhda Vyacheslavovna – Junior Researcher, Department Retraining and Improvement of Specialists. ORCID ID: 0000-0003-3743-6046
Kuzina Ekaterina Sergeevna – Junior Researcher, Department Retraining and Improvement of Specialists. ORCID ID: 0000-0001-5681-7616
Potapov Vasily Dmitrievich – Doctor of Biology, Chief Researcher, Department Retraining and Improvement of Specialists. ORCID ID: 0000-0002-5336-8234
Analysis of procurement of disinfectants in medicine facilities. Part 1. 2017–2018
D.V. Savraeva1, A.D. Salimova1, S.V. Andreev1, N.V. Shestopalov2
1Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F.F. Erisman: 18 Nauchny proezd, Moscow, 117246, Russian Federation;
2National Organization of Disinfectionists: Kashirskoe shosse 22, korp. 3, str. 2, Moscow, 115201, Russian Federation.
Disinfectants are very important in preventing infectious diseases and reducing the number of hospital-acquired infections. In recent years there has been a significant increase in the number of registered disinfectants. Such diversity requires from the end user a clear understanding of the properties of disinfectants, their effectiveness, toxicity, as well as chemical composition. Also an important aspect of the practical use of disinfectants is the quality control of working solutions, which makes at least familiarity with the basic chemical and analytical methods for the study of disinfectants. It is also necessary to know the features of regulation of disinfectants and to be aware of the latest scientific trends in this area. In this paper, we analyzed the documentation for auctions for the purchase of disinfectants by medical organizations of a wide profile in the Russian Federation between 2017 and 2019. Some common errors encountered during the compilation of disinfectant requirements are highlighted. It is shown that the most commonly used disinfectants based on cationic surfactants, alcohols, and halogens. At the same time, the most common active substance is hydrogen peroxide. Based on the analysis, recommendations on the preparation of requirements for disinfectants were given. The second part of this work will be dedicated to the COVID-19 pandemic disinfectant consumption in medical facilities.
Keywords: disinfectants, auctions, hospital-acquired infection.
Anna D. Salimova – junior researcher, Disinfectology Institute of F.F. Erisman FSCH of Rospotrebnadzor. ORCID ID: 0000-0002-4634-6977
Sergey V. Andreev – PhD in chemistry, leading researcher, head of chemical department, Disinfectology Institute of F.F. Erisman FSCH of Rospotrebnadzor. ORCID ID: 0000-0003-2405-9931
Nikolay V. Shestopalov – doctor of medicine sciences, professor, President of National Organisation of Disinfectionists. ORCID ID: 0000-0002-9973-3508
The use of unmanned aircraft to regulate the number of mosquitoes
S.A. Roslavtseva1, A.I. Zhulev2, D.A. Tsvetkov2, L.V. Medvedeva3
1Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F.F. Erisman: 18 Nauchny proezd, Moscow, 117246, Russian Federation;
2LLC «Gigiyena plyus»: 111B Mayakovskogo str., Anapa, Krasnodar Region, 353440, Russian Federation;
3Territorial department of the Office of the Federal Service for Supervision in the Field of Consumer Protection and Human Welfare in the Krasnodar Region in the resort city of Anapa: 1a Workers str., Anapa, Krasnodar Region, 353440, Russian Federation.
To improve the control measures against larvae of mosquitoes which are vectors of the most dangerous infectious diseases such as malaria, tularemia, various fevers (West Nile fever, yellow fever, dengue, Chikungunya, Zika and others) on the Black Sea coast of the Caucasus, unmanned aircraft «Odonata agro» 0501 and 1001 and «DJI Agras T-20» were used in 2020. Larvicides from the group of pyrethroids (cypermethrin), regulators of insect development (methoprene) and the microbiological formulation «Bactitsid» based on the entomopathogenic bacterium Bacillus thuringiensis var. israеlensis have shown the high efficacy when used by unmanned aircraft.
Keywords: mosquitoes, larvicides, unmanned aircraft.
Zhulev Anatoliy Ivanovich – Director of "Gigiyena plus"
Tsvetkov Dmitriy Aleksandrovich – Technical Director of "Gigiyena plus"
Medvedeva Larisa Vladimirovna – Head of the Maintenance Department of Rospotrebnadzor in the Krasnodar Territory in the resort city of Anapa
Prospects for the use of baits based on modern active ingredients against house flies
O.Yu. Eremina, V.V. Olifer, T.A. Davlianidze
Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F.F. Erisman: 8 Nauchny proezd, Moscow, 117246, Russian Federation.
Topical application revealed the high resistance of field populations of houseflies to pyrethroids (500–900×), neonicotinoids (345–500×), phenylpyrazoles (46–75×), susceptibility to FOS (0.6–1.4×) and oxadiazines (0.2×). With intestinal exposure, resistance rates are somewhat lower, but resistance remains at a high level to fipronil (33–76×), thiamethoxam (80–105×) and clothianidin (80–98×). Susceptibility to indoxacarb (0.2–1.4×) was revealed. The comparative efficacy of two modern baits based on indoxacarb and dinotefuran for resistant and susceptible strains of house flies was studied. Both insecticides are highly toxic to house flies in the form of commercially available baits. Indoxacarb and dinotefuran can be recommended for introduction into rotation schemes of insecticides in the form of food baits to control adult flies.
Keywords: dinotefuran, indoxacarb, house flies, insecticide resistance, baits.
Olifer Veronika Valentinovna – Candidate of Science in Biology; Leading Researcher of the Division of disinsection. ORCID ID: 0000-0002-4736-7905
Davlianidze Tatyana Alekseevna – Laboratory Research Assistant of the Division of disinsection. ORCID ID: 0000-0002-2757-881X
Information letter «Natural and focal infections, which agents are passed by ixodic ticks, and their nonspecific preventive measures in the Russian Federation (according to 01.01.2022)»
O.M. Germant1, M.B. Akhmetshina 1, V.A. Tsarenko2, E.V. Verigina2
1Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F.F. Erisman of Rospotrebnadzor, Moscow;
2Federal Center for Hygiene and Epidemiology of Rospotrebnadzor, Moscow.
Prevention of new coronavirus infection (COVID-19) in a specialized psychiatric hospital
M.I. Samoylov1, A.S. Pankov1, D.A. Kuzmina2, E.M. Kryukova2
1Federal State Budgetary Educational Institution of Higher Education «Orenburg State Medical University» of the Ministry of Health of the Russian Federation: 6 Soviet str., Orenburg, 460000, Russian Federation;
2State budgetary health care institution «Orenburg Regional Clinical Psychiatric Hospital № 1»: 5 Zwillinga str., Orenburg, 460006, Russian Federation.
At the end of 2019, an outbreak of a new coronavirus infection occurred in the People’s Republic of China (PRC), with its epicenter in the city of Wuhan (Hubei Province). The World Health Organization (WHO) has defined the official name of the infection caused by the novel coronavirus as Coronavirus disease 2019 («COVID-19») . The International Committee on the Taxonomy of Viruses assigned the official name of the causative agent of infection – SARS-CoV-2 . In accordance with the sanitary legislation of the Russian Federation, the virus is assigned to pathogenicity group II . COVID-19 occurs in two main forms that are subject to registration as independent nosological units: clinically pronounced – (U07.1) and carriage of the causative agent of coronavirus infection – (Z22.8) . All forms of manifestations of coronavirus infection are dangerous, but the asymptomatic form poses the greatest epidemiological danger, due to the difficulty of detection and, as a result, the untimely implementation of anti-epidemic measures . The role of COVID-19 as a healthcare-associated infection has been established . Sysin E.I. and co-authors established the maximum values of the foci index (IO) for nosocomial spread of COVID-19 in psychiatric hospitals . The inclusion of COVID-19 in the «List of diseases that pose a danger to others»  determines the epidemic significance of the disease.
Keywords: new coronavirus infection, anti-epidemic regime, psychiatric hospital, quality of anti-epidemic regime.
Pankov Aleksandr Sergeevich – Doctor of Medical Science, Head of the Department of Epidemiology and Infectious Diseases of The Orenburg State Medical University. ORCID ID: 0000-0003-4994-6633
Kuzmina Darya Aleksandrovna – Epidemiologistof Orenburg Regional Clinical Psychiatric Hospital No. 1
Kryukova Elena Mikhaylovna – Chief Physician of Orenburg Regional Clinical Psychiatric Hospital No. 1
Epidemiological characteristics of pertussis in the Orenburg region
I.V. Bozhenova, A.S. Pankov, V.V. Solovykh
Federal State Budgetary Educational Institution of Higher Education «Orenburg State Medical University» of the Ministry of Health of the Russian Federation: 6 Soviet str., Orenburg, 460000, Russian Federation.
The paper considers the relevance of the pertussis cough problem in the Orenburg region among different age groups. The manifestations of the epidemic process of pertussis in the long-term dynamics and among age groups for the period from 2005 to 2019 in the Orenburg region were studied and analyzed. A retrospective epidemiological analysis of the incidence of pertussis of the population was carried out, including an analysis of the long-term dynamics of the incidence of pertussis of the population, an analysis of the intra-annual dynamics of morbidity and an analysis of the long-term dynamics of morbidity among various population groups. It was found that during the study period, the average long-term incidence of pertussis in the Orenburg region was 1.0±0.21 per 100,000 population. The years with the registration of latent outbreak morbidity are highlighted – 2005, 2006 and 2019. The study established the years of the rise in morbidity – 2005, 2006, 2007, as well as 2017, 2018 and 2019. It is also established that all age groups of the child population are involved in the epidemic process of pertussis. The risk groups for the incidence of pertussis in Orenburg region are children under the age of 14 in the intra-annual dynamics of the incidence of whooping cough, spring-winter seasonality is noted.
Keywords: pertussis, Orenburg region, retrospective epidemiological analysis, risk areas, risk groups, time of risk, incidence.
Pankov Aleksandr Sergeevich – Doctor of Medical Science, Head of the Department of Epidemiology and Infectious
Manifestation of the epidemic process of coronavirus infection in the Republic of Moldova
Republican Psychiatric Hospital Balti: 114 Gagarin str., Balti, MD-3100, Republic of Moldova.
5 periods in the development of the epidemic process of COVID-19 infection have been identified. The epidemic process of coronavirus infection continues to develop unevenly with pronounced peaks in morbidity and mortality of the population of Moldova. In the first period (March–June 2020), 16613 cases of COVID-19 were reported, of which 545 were fatal (3.28 %). In the second period (July–December 2020) there is an increase of 5.4 times in the average monthly morbidity and 4.5 times in the average monthly mortality of the population from coronavirus infection. In the third period (January–March 2021), there was a slowdown in the intensity of the development of the epidemic process, but the monthly mortality rate of the population exceeded 1.5 times the indicator of the second period. The implementation of targeted specific and non-specific anti-epidemic measures contributed from April to October 2021 to a gradual reduction in the risk of coronavirus infection of the population. However, as a result of the cancellation of most restrictive anti-Covid measures, a decrease in the rate of immunization of the population, the intensification of migration processes and the import into the country by Moldovan citizens, on the eve of various holidays, over 4300 cases of COVID-19 infection, favorable conditions were created for the intensive development of the epidemic process of coronavirus infection and therefore the fifth period is characterized by the highest weekly morbidity rates (7813.8 cases) and mortality. (192.3 cases).
Keywords: coronavirus infection, biocidal preparations, epidemic process, population, seasonality, COVID-19 infection, morbidity, mortality, periods, peaks.
Life of National Organization of Disinfectionists (NOD)
Organization and conduct of final disinfection in centers of infectious diseases
L.I. Glushkova, I.V. Korabelnikov, Yu.I. Egorova
Evaluation of the efficiency of "St. Petersburg City Disinfection Station" over the past 10 years in the conditions of a new form of subordination
N.A. Vatolina, Yu.M. Shishkin, L.V. Lyalina
Congratulation new members of NOD
List of materials published in journal «Disinfection Affairs» in 2021