DISINFECTION
pp. 5-18
DOI: 10.35411/2076-457X-2025-2-5-18
Experimental selection of a test strain to evaluate the tuberculocidal efficacy of high-level disinfection of endoscopes. Part 1
N.I. Eremeeva1,2,3, A.S. Starikova1, F.A. Mukabenov1, A.V. Ilyakova1
1Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F.F. Erisman of the Federal Service for Supervision in Protection of the Rights of Consumer and Man Wellbeing: Nauchny proezd, 18 a, Moscow, 117 246, Russian Federation;
2NMRC of Tuberculosis and Infectious Diseases of Ministry of Health of the Russian Federation: 22 Partsyezda str., 50, Yekaterinburg, 620 039, Russian Federation;
3Federal State Budgetary Educational Institution of Further Professional Education "Russian Medical Academy of Continuous Professional Education" of the Ministry of Healthcare of the Russian Federation: Barrikadnay str., 2/1, Moscow, 125 993, Russian Federation.
The one of the priority tasks of disinfectology is the justified choice of test-microorganisms for evaluating the efficacy of disinfectants. Mycobacterium terrae DSM 43227 is the test-strain for assessing the tuberculocidal efficacy of disinfectants for disinfection of endoscopes, including high-level disinfection (HLD). However, it has been determined that M. terrae DSM 43227 were sensitive to the effect of heating during the drying stage with disinfection and washing machines (DWM) of HLD of endoscopes. This necessitated the search of isolate of mycobacterial for a promising as a test-strain with resistant to disinfectants and heat-resistant. At the first step of the study, the isolates of M. abscessus № 3, M. fortuitum № 2 и M. avium № 2, which have the highest resistance to disinfectants among the studied clinical isolates of fast- and slow-growing NTMBs (n = 14) were selected in comparison with test-strains (M. terrae DSM 43227; Mycobacterium B5; M. tuberculosis H37Rv) according to Guideline P 4.2.3676-20. At the second step, the sensitivity of selected isolates of NTMB and test-strains of mycobacterial to heating was studied. It has been established that M. fortuitum is a promising microorganism as test-strains of mycobacteria for assessing the efficiency of disinfectants for HLD of endoscopes with DWM. Because M. fortuitum has target resistance to the temperature factor (resistance to heating up to 60 °C for 1 hour), is resistant to chemical disinfectants and gives growth of visible colonies on nutrient media on average on 3–4 days of cultivation (which allows reducing the time for the study 5–7 times, from 21 days when using as a test-strain M. terrae DSM 43227 to 3–4 days when using M. fortuitum). In addition, the comparative assessment of resistance of clinical isolates of NTMB to disinfectants has revealed resistance of NTMB to tuberculocidal modes of application of disinfectants, which requires in-depth study, determination of clinical and epidemiological significance and search for approaches to ensure biological safety of susceptible population to infection with mycobacteriosis pathogens.
Keywords: high-level disinfection, flexible endoscopes, disinfectants, disinfection and washing machines, test-strain.
Authors
Natalya I. Eremeeva – Candidate of Biological Sciences, Head of the Department of Disinfection and Sterilization (with Microbiology Laboratory) of Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F.F. Erisman; assistant professor of the Department of Epidemiology and Disinfectology Federal State Budgetary Educational Institution of Further Professional Education "Russian Medical Academy of Continuous Professional Education" of the Ministry of Healthcare of the Russia; Leading Researcher of the NMRC of Tuberculosis and Infectious Diseases of Ministry of Health of the Russian Federation; e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID: 0000-0003-3637-2570
Anzhelica S. Starikova – Junior Researcher, Department of Disinfection and Sterilization, Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F.F. Erisman. ORCID ID: 0000-0002-5815-5916
Fedor А. Mukabenov – Researcher, Department of Disinfection and Sterilization, Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F.F. Erisman. ORCID ID: 0000-0002-5631-0775
Anastasia V. Ilyakova – Researcher, Department of Disinfection and Sterilization, Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F.F. Erisman. ORCID ID: 0000-0002-1867-3495
pp. 19-28
DOI: 10.35 411/2076-457X-2025-2-19-28
Determination of organic acids in disinfectants using ion-pair chromatography
A.D. Salimova1, A.A. Vasilieva1,2, T.D. Kugrysheva3, R.D. Solovov1,2
1Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F.F. Erisman of the Federal Service for Supervision in Protection of the Rights of Consumer and Man Wellbeing: Nauchny proezd, 18 a, Moscow, 117 246, Russian Federation;
2M.V. Lomosov Institute of Fine Chemical Technology of RTU-MIREA: 86 Vernadskogo ave., Moscow, 119 571, Russian Federation;3National University of Oil and Gas "Gubkin University": 65 Leninsky ave., Moscow, 119 991, Russian Federation.
The disinfection, pre-sterilisation cleaning and sterilisation of medical devices is aimed at preventing healthcare associated infections in patients and healthcare workers. Organic acid-based disinfectants are often used for pre-sterilisation cleaning and the product usually contains several active ingredients. Approximately 10 % of acid-based disinfectants contain three or more acids. Due to the similarity in chemical structure, the analysis of acids in disinfectants is difficult. This work demonstrates the possibility of using ion-pair chromatography for the determination of succinic, citric, salicylic, malic, malonic and ascorbic acids in disinfectants in the combined presence of succinic, citric, salicylic, malonic and ascorbic acids. The best separation of the mixture components was achieved using a Thermo Acclaim 120, C18, 5 μm (4.6 × 150 mm) analytical column. Acetonitrile was used as eluent A and 5 mM dodecyldimethylammonium chloride solution as eluent B, adjusted to pH 2.7 with H3PO4. The analysis was performed under gradient elution conditions. Detection was performed at wavelengths of 210–250 nm. Validation of the developed methodology was performed. The limit of detection of the proposed method is 26.76 mg/dm3 for citric acid, 20.09 mg/dm3 for malic acid, 0.66 mg/dm3 for ascorbic acid, 17.12 mg/dm3 for succinic acid, 5.89 mg/dm3 for malonic acid and 0.59 mg/dm3 for salicylic acid. Approval of the method on samples of disinfectants confirmed its high selectivity and the possibility of using it for simultaneous determination of a mixture of organic acids (malic, citric, ascorbic, malonic, succinic and salicylic acids) in disinfectants and working solutions.
Keywords: organic acids, disinfectants, HPLC, ion-pair chromatography.
Authors
Anna D. Salimova – Junior Researcher, Institute of Disinfectology of F.F. Erisman FSCH of Rospotrebnadzor; Tel.: (495) 586-11-44; e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID: 0000-0002-4634-6977
Alexandra A. Vasilieva – Laboratory Assistant ща Institute of Disinfectology of F.F. Erisman FSCH of Rospotrebnadzor, Student of the Department of Analytical Chemistry named after I.P. Alimarin of M.V. Lomosov Institute of Fine Chemical Technology of RTU-MIREA
Tatiana D. Kugrysheva – Student of the Department of Physical and Colloidal Chemistry of Gubkin State University
Roman D. Solovov – PhD in Chemistry, Senior Researcher of Institute of Disinfectology of F.F. Erisman FSCH of Rospotrebnadzor, assistant Professor of the Department of Analytical Chemistry named after I.P. Alimarin of M.V. Lomosov Institute of Fine Chemical Technology of RTU-MIREA. ORCID ID: 0000-0003-2452-0783
pp. 28-37
DOI: 10.35411/2076-457X-2025-2-28-37
Investigation of the effectiveness of disinfection of microbiological safety boxes with formalin, contaminated with smallpox vaccine virus
V.V. Solodky, V.V. Zolin, O.P. Os’kina, T.A. Gosteva
Federal Budgetary Institution of Science "State Scientific Center of Virology and Biotechnology "Vector" of the Federal Service for Supervision in the Field of Consumer Rights Protection and Human Well-Being: 630559, Russian Federation, Novosibirsk region, Koltsovo village.
To study the possibility of reducing the amount of formalin in the reaction mixture while maintaining 100 % disinfection efficiency, studies were conducted on the possibility of reducing the amount of formalin from 25 ml/m3 to 6.25 ml/m3 and studying the disinfecting efficiency of disinfecting solutions based on 40 % formalin with the addition of a 15 % solution of tertiary amines or a 3 % solution of chloramine in relation to the smallpox virus. This combination of disinfectants suggests a possible reduction in the toxic load on personnel during disinfection of a class 3 isolation box after work with pathogenic microorganisms in it. Objective. To compare the residual infectious activity of the smallpox vaccine virus after disinfection of the working chamber of the microbiological safety box (BMB) of the 3rd class of protection by evaporation on the flame of an alcohol lamp or in a chemical reaction with KMnO4 of different amounts, both pure 40% formalin and with the addition of a 15% solution of tertiary amines or a 3% solution of chloramine. Methods. The suspension method was used to determine the residual infectious activity of smallpox virus by TCID50/ml after exposure to combinations of disinfectants. The effectiveness of disinfection was assessed using metal tests in accordance with the requirements of the guideline P 4.2.3676-20 [18]. Results. The results of the studies showed a change in the titer of residual infectious activity of the smallpox virus during disinfection of biotests by evaporation of a combination of disinfectant solutions based on 40 % formalin with the addition of 15 % tertiary amine solution or 3 % chloramine solution. The use of different concentrations of 40 % formalin and its combinations with selected disinfectants made it possible to reduce the total amount of formalin in the evaporated reaction mixture by half, with the same disinfectant efficiency.
Keywords: disinfection, disinfection treatment, formalin, formaldehyde, fumigation.
Authors
Vladislav V. Solodky – Researcher at the Biosafety Department of the State Scientific Center of Virology and Biotechnology "Vector"; Tel.: (383) 363-47-00 (ext. 22-28), e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID: 0000-0002-7270-0028
Vladimir V. Zolin – Ph.D., Head of the Biosafety Department of the State Scientific Center of Virology and Biotechnology "Vector". ORCID ID: 0000-0003-4120-1178
Oksana P. Os’kina – Senior Researcher of the Biosafety Department of the State Scientific Center of Virology and Biotechnology "Vector". ORCID ID: 0000-0002-9165-1524
Tatyana A. Gosteva – Junior Researcher of the Biosafety Department of the State Scientific Center of Virology and Biotechnology "Vector". ORCID ID: 0009-0004-1508-9623
pp. 37-45
DOI: 10.35411/2076-457X-2025-2-37-45
DISINSECTION
Insecticidal activity of diamide-based food baits against the german cockroach
V.V. Olifer, O.Yu. Eremina
Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F.F. Erisman of the Federal Service for Surveillance on Consumer Rights otection and Human Wellbeing: 117246, Russian Federation, Moscow, Nauchniy proezd, 18a.
The efficacy of laboratory samples of baits based on diamides for the German cockroach of the insecticide-susceptible laboratory culture S-NIID and multi-resistant cultures MAG, KR, Amur-2, and OBN is shown. Higher resistance of females of resistant cultures of the German cockroach is revealed. The laboratory bait based on 0.1 % cyantraniliprole showed the best results. Males died completely, and 5–15 %females survived. The gel bait containig0.5 % cyantraniliprole was less attractive to the German cockroach. A high mortality rate of males (95–100 %) of all cultures was established, while the mortality rate of females was 95 % for S-NIID and from 30 % to 75 % for multi-resistant cultures. A decrease in the insecticidal effect of baits was established in the presence of alternative food. Flubendiamide in the form of 0.5 % food bait was not toxic to the German cockroach of all studied cultures.
Keywords: resistance, chlorantraniliprole, cyantraniliprole, flubendiamide, intestinal action, baits, Blattella germanica.
Authors
Olifer VeronikaValentinovna – PhD in Biological Sciences; Leading Researcher of the Division of disinsection (with laboratory of entomology) of the Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F.F. Erisman of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing. e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID: 0000-0002-4736-7905
Eremina Olga Yurevna – Doctor of Science in Biology; Leading Researcher of the Division of disinsection (with laboratory of entomology) of the Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F.F. Erisman of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing. ORCID ID: 0000-0002-8268-2230
DERATIZATION
pp. 45-57
DOI: 10.35411/2076-457X-2025-2-45-57
Analysis of the effectiveness of the rodenticide bait dosing mechanism at facilities of various industries
V.N. Timoshenkov1, T.B. Dubovik1, Yu.V. Protasova2
1Limited Liability Company "DEZ CENTER BRYANSK": 241004, Russian Federation, Bryansk region, Bryansk district, Bryansk region, Sven settlement, 4 Snezhetsky Val str., letter a, office 22;
2Limited Liability Company NPO "Garant": Russian Federation, 443 077, Samara, 180 Svobody str.
Scientific research and practice of controlling rodents indicate the need to take into account the state and properties of rodenticidal preparations used along with the biological characteristics of rodents. The present work is devoted to the problem of increasing the efficiency of rodent control methods in various industries, including agriculture. For this purpose, the authors conducted experiments on the application of the mechanism of rodenticide bait dosing and performed a comparative analysis of the effectiveness of deratisation measures in the conditions of application of the dosing mechanism and without it.
Keywords: dosing mechanism; rodenticide bait; rodent control.
Authors
Timoshenkov Vladimir Nikolaevich – Director of LLC "Dez Center Bryansk"; Tel.: (4832) 34-07-47, e-mail:Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID: 0009-0009-5373-2970
Dubovik Taras Borisovich – Disinfektologist of LLC "Dez Centr Bryansk". ORCID ID: 0009-0001-9757-9009
Protasova Yulia Vasilievna – Head of the Laboratory of LLC NPO "Garant". ORCID ID: 0009-0001-6712-9728
HYGIENE AND SANITATION
pp. 58-66
DOI:10.35411/2076-457X-2025-2-58-66
Assessment of the quality of drinking water in the city of Lipetsk
V.A. Bondarev1, S.S. Davydova1, Yu.V. Bespalova2
1FGBOU VO "Lipetsk State Pedagogical University named after P.P. Semenov-Tyan-Shansky": 398020, Russia, Lipetsk, Lenin St., 42;
2FGBOU VO "Ryazan State Medical University named after academician I.P. Pavlov": 390026, Ryazan, Vysokovoltnaya st., 9.
The results of studies of drinking water on organoleptic, chemical and microbiological indicators in 15 water intakes from 164 wells in the city of Lipetsk are presented. It was revealed that the total water hardness (mg-eq/kg) exceeds the MPC in water intakes № 2 and Syrsky mine, the chloride content (mg/ml) was reduced by more than 2 times in all water intakes, and the concentration of total iron (mg/ml) was increased in water intakes № 2 and TETs-2 by 13 %. A comparative analysis of the quality of water and its treatment methods showed that in the city of Lipetsk, a mechanical method is used for water treatment, with the help of which insoluble substances are eliminated from wastewater through settling systems and traps; reverse osmosis – separation of various aqueous substances under pressure through semi-permeable membranes; ozone filtration, during which ozone transfers dissolved impurities invisible to the eye in water into a suspension that is easily retained by washing filter loading; iron removal; water disinfection at treatment plants in Lipetsk is carried out using sodium hypochlorite, which is produced on membrane electrolysis plants from ordinary table salt. To minimize the content of nitrates in the water supply system, drinking water is mixed from various water intakes.
Keywords: drinking water, quality of drinking water, centralized water supply, Lipetsk.
Authors
Bondarev Vladimir Aleksandrovich – Doctor of Medical Sciences, Professor; Department of Physical Education, Physiology and Medical and Biological Disciplines of FGBOU VO "Lipetsk State Pedagogical University named after P.P. Semenov-Tyan-Shansky"; Tel.: (4742) 32-83-64, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID: 0000-0002-8578-8444
Davydova Selena Stanislavovna – Candidate of Biological Sciences, Associate Professor; Department of Physical Education, Physiology and Medical and Biological Disciplines of FGBOU VO "Lipetsk State Pedagogical University named after P.P. Semenov-Tyan-Shansky". ORCID ID: 0000-0001-9740-1465
Bespalova Yuliya Valerevna – Assistant; Department of Dermatovenereology and Laboratory Diagnostics of FGBOU VO "Ryazan State Medical University named after academician I.P. Pavlov". ORCID ID: 0009-0006-8276-6916
Life of National Organization of Disinfectionists (NOD)
pp. 66-69
Report on the conference "Organization of disinfection measures at epidemiologically significant (category) objects" (May 21-22, 2025, Tver)
p. 69
Congratulation new members of NOD