DISINFECTION
pp. 5-12
DOI: 10.35 411/2076-457X-2025-3-5-12
Study on toxicity of residual quantities of dishwashing detergent on white rats
M.V. Bidevkina1, M.V. Matrosenko1, A.I. Vinogradova1, E.N. Borkhunova2, M.A. Kuznetsova2, A.A. Usacheva2, Z.K. Shaikhutdinova1, A.D. Salimova1
1Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F.F. Erisman of the Rospotrebnadzor: 117246, Moscow, Nauchny proyezd, 18, Russian Federation;
2Moscow State Academy of Veterinary Medicine and Biotechnology – K.I. Skryabin Moscow Veterinary Academy: 109472, Moscow, Akademika Skryabina, 23, Russian Federation.
Incorrect use of detergents, despite the relative safety of their individual components, can have an adverse effect on humans. The toxicity of residual quantities of detergent was studied on rats with its intragastric administration in doses of 2.5–0.0025 mg/kg per day for 3 months. The doses were selected taking into account the permissible content of surfactants in water (0.5 mg/l). After 3 months, the rat blood serum was analyzed for alanine aminotransferase (ALT), aspartate aminotransferase, alkaline phosphatase, lactate dehydrogenase, cholinesterase, creatine phosphokinase, glucose, total protein, albumin, urea, creatinine, total bilirubin, cholesterol, low- and high-density lipoproteins (LDL and HDL), triglycerides, and phospholipids. The liver was histologically examined, internal organs were pathomorphologically examined, and their mass coefficients were determined. The detergent in the maximum tested doses of 2.5 mg/kg and 0.25 mg/kg caused an increase in ALT activity and changes in lipid metabolism: the detergent in a dose of 2.5 mg/kg caused a decrease in LDL and HDL, while in a dose of 0.25 mg/kg it caused only a decrease in HDL. Histopathological studies revealed destruction of the blood vessel walls, for all groups in varying degrees of severity. Signs of protein dystrophy and minor cicatricial changes of the liver predominated in rats that received the detergent in the highest doses (2.5 mg/kg and 0.25 mg/kg).
Keywords: toxicity, detergent, rats, histology, liver, lipid metabolism.
Authors
Bidevkina Marina Vasilevna – Doctor of Medical Sciences, Head of the Department of Toxicology (with laboratory) in Disinfectology Institute of the Federal Scientific Center of Hygiene named after F.F. Erisman of the Rospotrebnadzor; Phone: (495) 586-11-44 (# 1438); е-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID: 0000-0001-6433-899X
Matrosenko Margarita Vladimirovna – Junior Researcher, Department of Toxicology (with laboratory) in Disinfectology Institute of the Federal Scientific Center of Hygiene named after F.F. Erisman of the Rospotrebnadzor. ORCID ID: 0000- 0003-0567-2247
Vinogradova Arina Igorevna – Research Associate of the Department of Toxicology (with laboratory) in Disinfectology Institute of the Federal Scientific Center of Hygiene named after F.F. Erisman of the Rospotrebnadzor. ORCID ID: 0000-0002-3253-4571
Borkhunova Elena Nikolaevna – Doctor in Biological Sciences, Professor, Head of the Department of Anatomy and Histology named after Prof. A.F. Klimov of the Moscow State Academy of Veterinary Medicine and Biotechnology - K.I. Skryabin Moscow Veterinary Academy. ORCID ID: 0000-0003-2355-8616
Kuznetsova Maria Aleksandrovna – Assistants of the Department of Anatomy and Histology named after Prof. A.F. Klimov of the Moscow State Academy of Veterinary Medicine and Biotechnology - K.I. Skryabin Moscow Veterinary Academy. ORCID ID: 0000-0002-2031-1290
Usacheva Angelina Alekseevna – Assistants of the Department of Anatomy and Histology named after Prof. A.F. Klimov of the Moscow State Academy of Veterinary Medicine and Biotechnology - K.I. Skryabin Moscow Veterinary Academy. ORCID ID: 0000-0001-9460-3491
Shaykhutdinova Zukhra Kamilovna – Junior Researcher, Department of Toxicology (with laboratory) in Disinfectology Institute of the Federal Scientific Center of Hygiene named after F.F. Erisman of the Rospotrebnadzor. ORCID ID: 0000-0002-6364-9603
Salimova Anna Dmitrievna – Junior Researcher, Department of Chemical Research (with laboratory of analytical methods) in Disinfectology Institute of the Federal Scientific Center of Hygiene named after F.F. Erisman of the Rospotrebnadzor. ORCID ID: 0000-0002-4634-6977
pp. 13-21
DOI: 10.35 411/2076-457X-2025-3-13-21
Study of hydrogen peroxide decomposition dynamics at combined method of water disinfection (part 2)
A.S. Morozov, G.P. Pankratova, Z.K. Shaykhutdinova, V.A. Novikov
Disinfectology Institute of the Federal Scientific Center of Hygiene named after F.F. Erisman of Rospotrebnadzor: 117246, Moscow, Nauchny proyezd, 18, Russian Federation.
An experimental evaluation of the effectiveness of a combined method for disinfecting swimming pool water was conducted. Using test strains of spore- and non-spore-forming microorganisms as an example, it was found that the combined effect of ultraviolet radiation and hydrogen peroxide ensures their effective inactivation. Thus, treatment of water containing 0.01 % hydrogen peroxide with ultraviolet radiation led to complete inactivation of E. coli within 20 minutes. When exposed to UV radiation and 0.1 % hydrogen peroxide, the death of S. aureus in water was observed within 2 minutes. Inactivation of B. cereus spores by a combined method was achieved with a hydrogen peroxide content of 0.1 % in water and an exposure time of 45 minutes. The dose of UV radiation required for photolysis of hydrogen peroxide contained in water in a 0.1 % solution was 6888.8 J/cm2, and in a 0.01 % solution of PV – 4477 J/cm2. Ultraviolet radiation in combination with hydrogen peroxide is an effective method of disinfecting water from spore- and non-spore-forming forms of microorganisms, also ensuring the complete decomposition of hydrogen peroxide contained in water.
Keywords: swimming pool, water, combined disinfection method, hydrogen peroxide, ultraviolet radiation, test microorganism, efficiency.
Authors
Morozov Aleksandr Sergeevich – PhD in technical sciences, Senior Researcher of the Department of Toxicology in Disinfectology Institute of the Federal Scientific Center of Hygiene named after F.F. Erisman of Rospotrebnadzor; Tel.: (495) 582-92-40; e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID: 0009-0007-1852-5488
Pankratova Galina Pavlovna – PhD in medical sciences, Leading Researcher of the Department of Toxicology in Disinfectology Institute of the Federal Scientific Center of Hygiene named after F.F. Erisman of Rospotrebnadzor. ORCID ID: 0000-0003-2471-7906
Shaykhutdinova Zukhra Kamilovna – Junior Researcher at the Department of Toxicology in Disinfectology Institute of the Federal Scientific Center of Hygiene named after F.F. Erisman of Rospotrebnadzor. ORCID ID: 0000-0002-6364-9603
Novikov Vyacheslav Alekseevich – Junior Researcher at the Department of Disinfection in Disinfectology Institute of the Federal Scientific Center of Hygiene named after F.F. Erisman of Rospotrebnadzor. ORCID ID: 0000-0001-8858-5872
DISINSECTION
pp. 22-30
DOI: 10.35411/2076-457X-2025-3-22-30
New insecticides: study of intestinal effect on synanthropous insects
V.V. Olifer, O.Yu. Eremina
Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F.F. Erisman of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing: 117246, Russian Federation, Moscow, Nauchniy proezd, 18a.
The insecticidal properties of food baits based on four compounds were studied: fluralaner (isoxazolines), ethiprole (phenylpyrazoles), cyantraniliprole (diamides) and indoxacarb (oxadiazines) for males and females of the German cockroach of the susceptible S-NIID culture, the multiresistant M10 strain and the housefly of the susceptible S-NIID strain. For both cultures of the German cockroach, high efficiency of baits of 0.2–0.5 % fluralaner was demonstrated, providing 95–100 % efficiency on the 2nd day of the experiment, which indicates the possibility of its use in medical disinsection. The bait based on 0.5 % cyantraniliprole provided 95 % efficiency for the S-NIID culture on the 1st day of the experiment against male German cockroaches, however, females were more resistant and 80.0 ± 9.2 % of individuals died after 7 days. The bait had similar efficiency for the M10 culture, indicating the absence of cross-resistance. Consumption of the bait by houseflies for 24 hours provided efficiency of 98.3 ± 1.2 %. The bait based on 0.5 % indoxacarb is highly effective against the S-NIID German cockroach and the multiresistant M10 culture, providing after 2 days the death of 85–100 % and 80–95 % of insects, respectively. The effect of the bait on houseflies was somewhat slower and after 48 hours it provided the death of 89.4 ± 2.5 % of insects. Baits based on 0.05–0.07 % ethiprole did not show sufficient efficiency against synanthropic insects. For cockroaches of the S-NIID culture, 50 % mortality was achieved for males and females on the 4th day, and 95 % mortality was achieved only for males on the 7th day of the experiment. Ethiprole was not effective against the multiresistant culture of the German cockroach M10, and was low against the housefly. When counting after 72 hours, the mortality of adult flies when eating bait based on 0.05 % ethiprole was 35.8 ± 4.3 %, based on 0.07–66.7 ± 4.1 %. High resistance of females of both sensitive and resistant cultures of the German cockroach was revealed. For houseflies, all baits were highly effective except the ethiprole-based bait.
Keywords: Blattella germanica, Musca domestica, fluralaner, ethiprole, cyantraniliprole, indoxacarb, baits, resistance.
Authors
Olifer VeronikaValentinovna – PhD in Biological Sciences; Leading Researcher of the Division of disinsection (with laboratory of entomology) of the Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F.F. Erisman of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing. e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID: 0000-0002-4736-7905
Eremina Olga Yurevna – Doctor of Science in Biology; Leading Researcher of the Division of disinsection (with laboratory of entomology) of the Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F.F. Erisman of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing. ORCID ID: 0000-0002-8268-2230
VIROLOGY
pp. 31-40
DOI: 10.35411/2076-457X-2025-3-31-40
Updated collection of test strains viruses and bacteria of the Institute of disinfectantology F.F. Erisman federal scientific centre of hygiene of Ropotrebnadzor, as an important tooi for increasing efficiency of modern disinfectant agents and medical technologies
Yu.A. Zakharova
F.F. Erisman Federal Scientific Centre of Hygiene: 141014, Russian Federation Moscow region, Mytishchi, Semashko str. 2.
The article discusses the state of the collection of test strains of viruses and bacteria at the Institute of Disinfection and the need to update the existing collection. It is known that the main requirements for test strains are their intended purpose, availability for testing, accuracy, and reproducibility of results. Currently, not all test strains meet these requirements, which suggests a change in the methodological approaches to their selection. Bacteria strains from the ESCAPE pathogen group, which have a high level of resistance to disinfectants, can be considered promising candidates for assessing the bactericidal activity and effectiveness of disinfection agents and technologies in medical organizations. Due to the significant limitation of the use of the polio virus in the next 5–10 years, in accordance with the WHO Global Action Plan, the poliovirus test strain (LSc2ab vaccine strain), which is currently used to assess the virucidal activity of disinfectants, as well as to control water disinfection by photocatalysis, requires replacement. in the first case, non-polio enteroviruses, in the second, including monitoring the effectiveness of air disinfection, bacteriophages, in particular phages with single-stranded RNA. Improving the methods of assessing disinfection agents and technologies using new test strains of viruses and bacteria with optimal target characteristics will allow for higher-level disinfection expertise, providing competitive advantages to conscientious manufacturers in the product market, and improving the quality of medical care in healthcare facilities, leading to a decrease in infectious diseases and overall reduction of biological risks.
Keywords: test strains of microorganisms, working collection, viruses, bacteria, disinfectants, medical technologies.
Author
Zakharova Yulia Alexandrovna – Doctor of Medical Sciences, Professor, Scientific Supervisor in Institute of Disinfectology of FBIS F.F. Erisman of Federal Scientific Centre of Hygiene Federal Service for Surveillance on Costumer Rights Protection and Human Wellbeing; Tel.: (495) 582-92-40, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID: 0000-0003-3416-0902
DERATIZATION
pp. 41-49
DOI: 10.35411/2076-457X-2025-3-41-49
Results of surveillance of the state of small mammal population of stationary observation posts territory of the Khabarovsk krai and epidemiologically significant objects of the Khabarovsk city in 2024
D.N. Poleshchuk1, A.G. Kovalsky1, A.G. Dragomeretskaya2, O.E. Trotsenko2, T.A. Pomortseva1
1FKUZ “Khabarovsk anti-plague station" of the Federal service for surveillance on consumers rights protection and human wellbeing: 680031, Khabarovsk, Sanitary lane, 7, Russian Federation;
2FBUN "Khabarovsk research institute of epidemiology and microbiology" of the Federal service for surveillance on consumers rights protection and human wellbeing: 680000, Khabarovsk, Shevchenko street, 2, Russian Federation.
The obtained data on zoological surveillance over small mammal population of three stationary observation posts located in the Khabarovsk krai and epidemiologically significant objects of the of the Khabarovsk city are presented. Meteorological conditions of the year 2024 were mostly favorable for small mammals in the surveyed territory. At the same time, changes of dominant and codominant species were noted in different observation posts. Positive reproductive rates of non-dominant animal species recorded in 2024 give reason to assume that the number of small mammals in all surveyed posts of the Khabarovsk krai will increase by 5–15 %. No changes is small mammals population is expected in the Lazo district of the Khabarovsk krai or it might increase by 5–15 %.
Keywords: small mammals, natural focal infections, surveillance over population status, Khabarovsk krai, non-dominant species.
Authors
Poleshchuk Dmitriy Nikolaevich – Head of the Zoological and Parasitological Laboratory of Khabarovsk anti-plague station; Tel.: (4212) 33-44-88, e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.. ORCID ID: 0000-0003-3964-2724
Kovalskiy Andrey Grigorevich – Candidate of Medical Sciences, Director of Khabarovsk anti-plague station. ORCID ID: 0009-0000-3173-6254
Dragomeretskaya Anna Gennadevna – Candidate of Biological Sciences, Head of the Department of Natural Focal Infections of Khabarovsk research institute of epidemiology and microbiology. ORCID ID: 0000-0003-1829-1849
Trotsenko Olga Evgenevna – Doctor of Medical Sciences, Director of Khabarovsk research institute of epidemiology and microbiology. ORCID ID: 0000-0003-3050-4472
Pomortseva Tatyana Anatolevna – Zoologist of Khabarovsk anti-plague station. ORCID ID: 0009-0006-9291-6076
HISTORY OF DISINFECTOLOGY
pp. 50-57
DOI: 10.35411/2076-457X-2025-3-50-57
Scientific and practical activities of the founder of disinfection, Ya.L. Okunevsky: on the 85th anniversary of his death
N.A. Vatolina1, V.G. Erofeev2
1FBUN "St. Petersburg Scientific Research Institute of Epidemiology and Microbiology named after Pasteur" of Rospotrebnadzor: 197101, St. Petersburg, Mira st., 14, Russian Federation;
2FGBVOU VO "Military Medical Academy named after S.M. Kirov" of the Ministry of Defense of the Russian Federation: 194044, St. Petersburg, Akademika Lebedeva st., 6Zh, Russian Federation.
In 2025, it will be 85 years since the death of Yakov Leontievich Okunevsky, an Honored Scientist of the RSFSR, a student of Professor V.A. Levashev, Head of the Department of Microbiology and Epidemiology with an independent course on disinfection at the S.M. Kirov Military Medical Academy, and the founder of disinfection in Leningrad. There are no publications in the modern scientific literature dedicated to his scientific and public activities. This article is based on the results of work with archival funds and literary sources. From 1918, when the Disinfection and Repair Brigade was established, until his death, Okunevsky served as the permanent assistant to the head of the Brigade, P.A. Patsanovsky, acting as his deputy for scientific and methodological issues. Yakov Leontievich actively combined his scientific work with his involvement in the Red Army and his civilian life. Keywords: history of preventive medicine, disinfection, history of hygiene, Military Medical Academy, city disinfection station, V.A. Levashev, P.A. Patsanovsky, V.G. Khlopin, A.A. Smorodintsev.
OBITUARY
p. 58
Veselov A.A.
Life of National Organization of Disinfectionists (NOD)
p. 30
Congratulation new members of NOD
ADVERTISEMENT
pp. 59-63
V.N. Timoshenkov, T.B. Dubovik
Automation of accounting of measures and reporting to control and supervisory authorities during disinsection and deratization measures
Authors
Vatolina Natalya Anatolyevna – Degree Applicant of FBUN "St. Petersburg Research Institute of Epidemiology and Microbiology named after Pasteur" of Rospotrebnadzor; e-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.
Erofeev Vitaliy Grigorevich – Candidate of Medical Sciences, Associate Professor; Associate Professor of the Department of General and Military Hygiene with a Course of Naval and Radiation Hygiene of Military Medical Academy named after S.M. Kirov